Expression of hui^GD4540 under the control of Scer\GAL4^ptc-559.1 results in stubby wings, narrows the L3-L4 intervein region and reduces the anterior crossvein. Gaps in L3 are also seen. Expression throughout the wing using Scer\GAL4^C-765 results in small wings and causes L3-L4 malformation and occasional partial ablation of the anterior crossvein. A small posterior compartment is seen when hui^GD4540 is expressed under the control of Scer\GAL4^en-e16E. The L3-L4 intervein region is not reduced and the anterior crossvein is present. However the phasing of the bristles at the posterior wing margin is disturbed.

Adults expressing hui^GD4540 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD4540} insertion line used.

Expression of hui^GD4540 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD4540} insertion line used.

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 20-30% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 20-30% of the Scer\GAL4^pnr-MD237-expressing area of the notum.