FlyBase Allele Report: Dmel\Dmtn[GD2834]

General Information

Symbol

Dmel\Dmtn^GD2834

Species

D. melanogaster

Name

FlyBase ID

FBal0198947

Feature type

allele

Associated gene

Dmel\Dmtn

Associated Insertion(s)

Carried in Construct

P{GD2834}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD2834}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD2834

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^NP6590

(Hopkins, 2013)

abnormal neuroanatomy | adult stage, with Scer\GAL4^repo

(Hopkins, 2013)

partially lethal - majority die, with Scer\GAL4^NP6590

(Hopkins, 2013)

partially lethal - majority die, with Scer\GAL4^repo

(Hopkins, 2013)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

adult optic lobe, with Scer\GAL4^NP6590

(Hopkins, 2013)

adult optic lobe, with Scer\GAL4^repo

(Hopkins, 2013)

medulla, with Scer\GAL4^NP6590

(Hopkins, 2013)

medulla anlage, with Scer\GAL4^repo

(Hopkins, 2013)

Detailed Description

Statement

Reference

Flies expressing Dmtn^GD2834 in the glia under the control of Scer\GAL4^repo are semi-lethal; ~20% of larvae eclose. The optic lobes are severely reduced in size, and missing much of the medulla neuropil. The same phenotypes are observed when Dmtn^GD2834 is expressed under the control of Scer\GAL4^Dmtn-NP6590, with 90.4% of flies failing to eclose. The optic lobe defects are weaker than under the control of Scer\GAL4^repo. No lethality or optic lobe defects are observed when Dmtn^GD2834 is expressed in neurons under the control of Scer\GAL4^elav-C155.

10 and 30 day old flies expressing Dmtn^GD2834 under the control of Scer\GAL4^elav-C155 have normal lifespans compared to controls.

(Hopkins, 2013)

Adults expressing Dmtn^GD2834 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2834

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Dmtn^GD2834, Scer\GAL4^elav-C155 is an enhancer of visible phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav-C155

(Hopkins, 2013)

Suppressor of

Statement

Reference

Dmtn^GD2834, Scer\GAL4^elav-C155 is a suppressor of partially lethal - majority die phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav-C155

(Hopkins, 2013)

Phenotype Manifest In

Enhancer of

Statement

Reference

Dmtn^GD2834, Scer\GAL4^elav-C155 is an enhancer of wing phenotype of Hsap\APP^{695.UAS.Tag:MYC}, Scer\GAL4^elav-C155

(Hopkins, 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Dmtn^GD2834 partially suppresses the predominantly male semi-lethality seen when Hsap\APP^{695.Scer\UAS.T:Hsap\MYC} is expressed in neurons under the control of Scer\GAL4^elav-C155 (co-expressed with Dcr-2^Scer\UAS.cDa). The wing expansion defects are enhanced compared to expression of Dmtn^GD2834 alone.

(Hopkins, 2013)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v37336

w¹¹¹⁸; P{GD2834}v37336

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2834

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG1021^GD2834

Dmtn^GD2834

Tmcc^GD2834

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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