FlyBase Allele Report: Dmel\Srpk79D[GD2777]

General Information

Symbol

Dmel\Srpk79D^GD2777

Species

D. melanogaster

Name

FlyBase ID

FBal0199452

Feature type

allele

Associated gene

Dmel\Srpk79D

Associated Insertion(s)

Carried in Construct

P{GD2777}

Also Known As

UAS-Srpk79D-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD2777}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD2777

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

abnormal smell perception | adult stage, with Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

adult olfactory receptor neuron, with Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

presynaptic active zone | adult stage, with Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

synapse | adult stage, with Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

Detailed Description

Statement

Reference

Expression of Srpk79D^GD2777 under the control of Scer\GAL4^peb-GAL4 results in the decreased decreased synapse number (assessed by counting active zone marker puncta) in the VA1v olfactory receptor neurons in adult flies and in a complete loss of olfactory attraction of adult flies to an attractive odorant (apple cider vinegar) in a trap assay.

(Mosca et al., 2017)

Adults expressing Srpk79D^GD2777 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2777

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^peb-GAL4, Srpk79D^GD2777 has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Lrp4^{UAS.Tag:HA,Tag:FLAG}, Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

Suppressor of

Statement

Reference

Scer\GAL4^da.PU/Srpk79D^GD2777, Scer\GAL80^ts.αTub84B is a suppressor | heat sensitive of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^peb-GAL4, Srpk79D^GD2777 has adult olfactory receptor neuron phenotype, non-suppressible by Lrp4^{UAS.Tag:HA,Tag:FLAG}, Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

Scer\GAL4^peb-GAL4, Srpk79D^GD2777 has synapse | adult stage phenotype, non-suppressible by Lrp4^{UAS.Tag:HA,Tag:FLAG}, Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

Scer\GAL4^peb-GAL4, Srpk79D^GD2777 has presynaptic active zone | adult stage phenotype, non-suppressible by Lrp4^{UAS.Tag:HA,Tag:FLAG}, Scer\GAL4^peb-GAL4

(Mosca et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The decreased synapse number (assessed by counting active zone marker puncta) in the VA1v olfactory receptor neurons observed in adult flies expressing Srpk79D^GD2777 under the control of Scer\GAL4^peb-GAL4 cannot be overcome by concurrent expression of Lrp4^{Scer\UAS.T:Ivir\HA1,T:Zzzz\FLAG}.

(Mosca et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v47544

w¹¹¹⁸; P{GD2777}v47544

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2777

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG11489^GD2777

Srpk79D^GD2777

srpk79D^GD2777

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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