FlyBase Allele Report: Dmel\Evi5[GD6735]

General Information

Symbol

Dmel\Evi5^GD6735

Species

D. melanogaster

Name

FlyBase ID

FBal0199536

Feature type

allele

Associated gene

Dmel\Evi5

Associated Insertion(s)

Carried in Construct

P{GD6735}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD6735}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD6735

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration, with Scer\GAL4^c306

(Laflamme et al., 2012)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

border follicle cell, with Scer\GAL4^c306

(Laflamme et al., 2012)

Detailed Description

Statement

Reference

Knockdown of Evi5 in border cells, through the expression of Evi5^GD6735 under the control of Scer\GAL4^c306, induces border cell migration defects.

Expression of Evi5^GD6735 under the control of Scer\GAL4^c306 slightly decreases the volume distribution of sec15-positive structures at the leading edge of border cells, while increasing the size of the individual vesicles.

Expression of Evi5^GD6735 under the control of Scer\GAL4^c306 slightly increases the volume distribution of Rip11-positive structures at the leading edge of border cells, while decreasing the size of the individual vesicles.

(Laflamme et al., 2012)

Expression of Evi5^GD6735 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver results in viable flies or partial lethality, depending on the particular P{GD6735} insertion line used.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development