Expression of wash^GD7950 under the control of Scer\GAL4^{VP16.mat.αTub67C} leads to disrupted actin organization at outer ring canals in stage 7-9 egg chambers, premature ooplasmic streaming in stage 7 oocytes and disrupted cortical actin organization in stage 7-9 oocytes when compared to controls.

The expression of wash^GD7950 under the control of Scer\GAL4^Hml.Δ leads to macrophages presenting decreased cell spread area on uncoated glass surface, as compared to controls.

Hemocyte trans-migration from the head to the tail is similar in embryos expressing wash^GD7950 under the control of Scer\GAL4^Pxn.PS and wild-type. However, compared with wild-type, expression of Scer\GAL4^Pxn.PS>wash^GD7950 significantly reduces the number of hemocytes that migrate anteriorly from the tail along the ventral midline.

Compared with wild-type, posterior hemocytes in Scer\GAL4^Pxn.PS>wash^GD7950 embryos exhibit severe reduction in cellular protrusion area. The anterior hemocytes of the same genotype also show a moderate reduction in cellular protrusion area. The number of vacuoles present in both the anterior and posterior hemocyte cell bodies is increased relative to wild-type.

Adults expressing wash^GD7950 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Oocytes of females expressing wash^GD7950 under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} undergo premature cytoplasmic streaming. The normal gradient of microtubule organisation that is seen in wild-type oocytes at stage 7 is lost in the mutant oocytes. The mutant stage 7 oocytes have disruptions in the cortical actin.