FlyBase Allele Report: Dmel\haf[GD14353]

General Information

Symbol

Dmel\haf^GD14353

Species

D. melanogaster

Name

FlyBase ID

FBal0200755

Feature type

allele

Associated gene

Dmel\haf

Associated Insertion(s)

Carried in Construct

P{GD14353}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14353}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14353

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

abnormal neuroanatomy, with Scer\GAL4^G14

(Kurusu et al., 2008)

abnormal neuroanatomy, with Scer\GAL4^how-24B

(Kurusu et al., 2008)

abnormal neuroanatomy, with Scer\GAL4^l(3)H94-H94

(Kurusu et al., 2008)

abnormal neuroanatomy, with Scer\GAL4^tey-5053A

(Kurusu et al., 2008)

abnormal neuroanatomy, with Scer\GAL4^Tub.PU

(Kurusu et al., 2008)

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

lethal, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal - all die during embryonic stage, with Scer\GAL4^Tub.PU

(Kurusu et al., 2008)

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during embryonic stage, with Scer\GAL4^Tub.PU

(Kurusu et al., 2008)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

Phenotype Manifest In

larval intersegmental nerve branch ISNb of A1-7, with Scer\GAL4^G14

(Kurusu et al., 2008)

larval intersegmental nerve branch ISNb of A1-7, with Scer\GAL4^how-24B

(Kurusu et al., 2008)

larval intersegmental nerve branch ISNb of A1-7, with Scer\GAL4^l(3)H94-H94

(Kurusu et al., 2008)

larval intersegmental nerve branch ISNb of A1-7, with Scer\GAL4^tey-5053A

(Kurusu et al., 2008)

larval ventral nerve cord, with Scer\GAL4^elav.PLu

(Kurusu et al., 2008)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Adults expressing haf^GD14353 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) can show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies, depending on the particular P{GD14353} insertion line used.

Adults expressing haf^GD14353 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show no significant change in the kinetics of high temperature (46[o]C) induced paralysis compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or late larval lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage or semi-lethality at the pupal stage, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 70-80% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 10-20% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

Stage 17 haf^GD14353; Scer\GAL4^how-24B embryos display a range of defects in the trajectory of the ISNb with a penetrance of over 60%. ISNb defects include truncation, bifurcation, bypassing the ventral-lateral muscle field (the normal targets for innervation by ISNb), targeting of the wrong muscles and fusion with the transverse nerve. Similar phenotypes are seen at a lower penetrance when Scer\GAL4^G14 is used in place of Scer\GAL4^how-24B, and are seen in larvae when Scer\GAL4^l(3)H94-H94 or Scer\GAL4^5053A is used. haf^GD14353; Scer\GAL4^5053A larvae have ectopic ISNb branches projecting to muscle 5 or 8.

haf^GD14353; Scer\GAL4^αTub84B.PL is embryonic lethal. When insertion P{GD14353}v36220 is used, haf^GD14353; Scer\GAL4^how-24B is larval lethal. P{GD14353}v36220 Scer\GAL4^elav.PLu embryos have defects in the commissure ladder of the ventral nerve cord.

(Kurusu et al., 2008)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14353

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v36220

w¹¹¹⁸; P{GD14353}v36220

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14353

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG14351^GD14353

haf^GD14353

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close