Expression of Ir56d^GD2096 under the control of Scer\GAL4^Gr64f.9.7 in adults results in proboscis extension rates that are significantly reduced in response to fatty acids, but not in response to sucrose, compared to controls.

Expression of Ir56d^GD2096 under the control of Scer\GAL4^elav.PU (in combination with a Dicer-2 transgene to enhance RNAi efficiency) does not lead to a significant change in female feeding preference index for 1% yeast extract versus 5mM sucrose solution, as compared to controls.

In a feeding preference assay for amino-acid-depleted yeast extract supplemented with serine, threonine and alanine, females expressing Ir56d^GD2096 under the control of Scer\GAL4^elav.PU (in combination with a Dicer-2 transgene to enhance RNAi efficiency) exhibit a significantly decreased preference index, as compared to controls.

Expression of Ir56d^GD2096 under the control of Scer\GAL4^Gr33a-GAL4 has no impact on the L-canavanine avoidance seen in wild type flies in a two-way choice behavioral assay.

Adults expressing Ir56d^GD2096 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.