FlyBase Allele Report: Dmel\Vlet[GD5216]

General Information

Symbol

Dmel\Vlet^GD5216

Species

D. melanogaster

Name

FlyBase ID

FBal0202306

Feature type

allele

Associated gene

Dmel\Vlet

Associated Insertion(s)

Carried in Construct

P{GD5216}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD5216}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD5216

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^Mef2.PR

(Lanfranco et al., 2017)

abnormal pain response | adult stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

lethal, with Scer\GAL4^c179

(Lanfranco et al., 2017)

lethal, with Scer\GAL4^da.G32

(Lanfranco et al., 2017)

lethal, with Scer\GAL4^how-24B

(Lanfranco et al., 2017)

lethal, with Scer\GAL4^αTub84B.PL

(Lanfranco et al., 2017)

partially lethal, with Scer\GAL4^elav-C155

(Lanfranco et al., 2017)

partially lethal, with Scer\GAL4^G7

(Lanfranco et al., 2017)

partially lethal, with Scer\GAL4^insc-Mz1407

(Lanfranco et al., 2017)

partially lethal, with Scer\GAL4^Mef2.PR

(Lanfranco et al., 2017)

partially lethal, with Scer\GAL4^RapGAP1-OK6

(Lanfranco et al., 2017)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of Glos^GD17786, together with UAS-Dicer2 to enhance RNAi efficiency, is lethal at both 25[o]C or 29[o]C if driven by Scer\GAL4^αTub84B.PL, Scer\GAL4^da.G32, Scer\GAL4^how-24B or Scer\GAL4^c179 and significantly reduced at both temperatures or just the higher one if driven by any of the following: Scer\GAL4^RapGAP1-OK6, Scer\GAL4^insc-Mz1407, Scer\GAL4^elav-C155, Scer\GAL4^G7 or Scer\GAL4^Mef2.PR. Without the UAS-Dicer2, the expression of Glos^GD17786 is completely lethal at both temperatures only with Scer\GAL4^αTub84B.PL and lethal at 29[o]C but with some flies surviving at 25[o]C with the Scer\GAL4^da.G32 driver. Adult viability is not strongly reduced if Glos^GD17786 is controlled by Scer\GAL4^RapGAP1-OK6, Scer\GAL4^insc-Mz1407, Scer\GAL4^elav-C155, Scer\GAL4^G7 or Scer\GAL4^Mef2.PR whereas Scer\GAL4^how-24B- or Scer\GAL4^c179-driven expression leads to partial lethality (especially at 29[o]C).

The climbing as well as flight ability of flies expressing Vlet^GD5216 (with UAS-Dicer2) under the control of either Scer\GAL4^Mef2.PR is strongly reduced compared to controls.

(Lanfranco et al., 2017)

Adults expressing CG2371^GD5216 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show significantly reduced avoidance of noxious temperature (46[o]C) compared to control flies.

Adults expressing CG2371^GD5216 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) show no significant change in the kinetics of high temperature (46[o]C) induced paralysis compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 30-40% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 10-20% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5216

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Gem3^ΔN.UAS, Scer\GAL4^Mef2.PR, Vlet^GD5216 has short lived phenotype

(Lanfranco et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Flies expressing simultaneously Vlet^GD5216 and Gem3^ΔN.Scer\UAS under the control of Scer\GAL4^Mef2.PR display significantly reduced survival rate compared to controls.

(Lanfranco et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v15482