FlyBase Allele Report: Dmel\smash[GD10286]

General Information

Symbol

Dmel\smash^GD10286

Species

D. melanogaster

Name

FlyBase ID

FBal0202910

Feature type

allele

Associated gene

Dmel\smash

Associated Insertion(s)

Carried in Construct

P{GD10286}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10286}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10286

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates tauopathy

modeled by Hsap\MAPT^R406W.UAS

(Butzlaff et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | larval stage, with Scer\GAL4^GMR.PS

(Tanaka et al., 2019)

increased occurrence of cell division | larval stage, with Scer\GAL4^GMR.PS

(Tanaka et al., 2019)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible | adult stage, with Scer\GAL4^GMR.PS

(Tanaka et al., 2019)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PS

(Tanaka et al., 2019)

eye disc, with Scer\GAL4^GMR.PS

(Tanaka et al., 2019)

Detailed Description

Statement

Reference

Expression of smash^GD10286 under the control of Scer\GAL4^GMR.PS (but not Scer\GAL4^pnr.PU, Scer\GAL4^dpp.PU or Scer\GAL4^Bx-MS1096) leads to a rough adult eye phenotype (more severe in males than in females). Its expression under the control of Scer\GAL4^GMR.PS also leads to increased apoptosis (Caspase 3 staining) as well as increased cell proliferation (in the posterior region to the synchronized S phase zone, EdU incorporation) in the eye imaginal discs while it doesn't lead to any changes in the differentiation of larval photoreceptor cells (prospero staining) or pupal cone cells (cut staining) when compared to controls.

(Tanaka et al., 2019)

Adults expressing CG43427^GD10286 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development