FlyBase Allele Report: Dmel\vito[GD10890]

General Information

Symbol

Dmel\vito^GD10890

Species

D. melanogaster

Name

FlyBase ID

FBal0203319

Feature type

allele

Associated gene

Dmel\vito

Associated Insertion(s)

Carried in Construct

P{GD10890}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10890}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10890

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^Ubi.PU

(Marinho et al., 2011)

abnormal size | adult stage, with Scer\GAL4^ey.PU

(Marinho et al., 2011)

abnormal size | third instar larval stage, with Scer\GAL4^ey.PU

(Marinho et al., 2011)

abnormal size | third instar larval stage, with Scer\GAL4^ptc-559.1

(Marinho et al., 2011)

decreased cell number | somatic clone | third instar larval stage, with Scer\GAL4^Act5C.PI

(Marinho et al., 2011)

decreased cell size | third instar larval stage, with Scer\GAL4^ptc-559.1

(Marinho et al., 2011)

increased cell death, with Scer\GAL4^ey.PU

(Marinho et al., 2011)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of vito^GD10890 using Scer\GAL4^ey.PU results in a reduced and rough adult eye. In the eye primordium, posterior retinal differentiation still occurs, but a very significant reduction in eye disc size is visible, particularly in the anterior proliferative region.

Scer\GAL4^Ubi-mediated expression of vito^GD10890 results in a significant developmental delay.

vito^GD10890-expressing clones induced early in larval development and analysed in third instar eye discs are significantly smaller than control clones, both in the peripodial epithelium and the eye disc proper. This is not due to a reduction in cell size.

Scer\GAL4^ey.PU, vito^GD10890 eye discs exhibit significantly more cells undergoing apoptosis compared to controls.

Scer\GAL4^ptc-559.1-mediated expression of vito^GD10890 results in reduced larval salivary gland growth. There is a substantial reduction in the packaging of nucleolar components and nucleoli exhibit an overall granular organization. Salivary gland cells are significantly smaller than controls owing to a reduction in cytoplasmic volume; nuclear volumes are not significantly reduced.

(Marinho et al., 2011)

Adults expressing vito^GD10890 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 80-90% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10890

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ey.PU, vito^GD10890 has abnormal size | third instar larval stage phenotype, suppressible | partially by Df(3L)H99/+

(Marinho et al., 2011)

Scer\GAL4^ey.PU, vito^GD10890 has increased cell death phenotype, suppressible by Df(3L)H99/+

(Marinho et al., 2011)

Scer\GAL4^ey.PU, vito^GD10890 has increased cell death phenotype, suppressible | partially by bsk^DN.UAS/Scer\GAL4^ey.PU

(Marinho et al., 2011)

Scer\GAL4^ey.PU, vito^GD10890 has abnormal size phenotype, suppressible by Hsap\NOL12^UAS.GFP, Scer\GAL4^ey.PU

(Marinho et al., 2011)

Scer\GAL4^ey.PU, vito^GD10890 has abnormal size | third instar larval stage phenotype, suppressible | partially by BacA\p35^UAS.cHa, Scer\GAL4^ey.PU

(Marinho et al., 2011)

Scer\GAL4^ey.PU, vito^GD10890 has increased cell death phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^ey.PU

(Marinho et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^ey.PU, vito^GD10890 has abnormal size | third instar larval stage phenotype, non-suppressible by p53^5A-1-4/p53^5A-1-4

(Marinho et al., 2011)

Scer\GAL4^ey.PU, vito^GD10890 has increased cell death phenotype, non-suppressible by p53^5A-1-4/p53^5A-1-4

(Marinho et al., 2011)

Scer\GAL4^ey.PU, vito^GD10890 has abnormal size | third instar larval stage phenotype, non-suppressible by p53^R155H.UAS.Ex/Scer\GAL4^ey.PU

(Marinho et al., 2011)

Scer\GAL4^ey.PU, vito^GD10890 has increased cell death phenotype, non-suppressible by p53^R155H.UAS.Ex/Scer\GAL4^ey.PU

(Marinho et al., 2011)

Scer\GAL4^ey.PU, vito^GD10890 has abnormal size | third instar larval stage phenotype, non-suppressible by bsk^DN.UAS/Scer\GAL4^ey.PU

(Marinho et al., 2011)

Suppressor of

Statement

Reference

vito^GD10890, Scer\GAL4^Act5C.PI is a suppressor | partially of increased cell size | somatic clone phenotype of Myc^UAS.cZa, Scer\GAL4^Act5C.PI

(Marinho et al., 2011)

vito^GD10890, Scer\GAL4^Act5C.PI is a suppressor of abnormal size | somatic clone phenotype of Myc^UAS.cZa, Scer\GAL4^Act5C.PI

(Marinho et al., 2011)

vito^GD10890, Scer\GAL4^ptc-559.1 is a suppressor of increased cell size phenotype of Myc^UAS.cZa, Scer\GAL4^ptc-559.1

(Marinho et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ey.PU, vito^GD10890 has eye disc phenotype, suppressible | partially by BacA\p35^UAS.cHa, Scer\GAL4^ey.PU

(Marinho et al., 2011)

Scer\GAL4^ey.PU, vito^GD10890 has eye disc phenotype, suppressible | partially by Df(3L)H99/+

(Marinho et al., 2011)

Scer\GAL4^ey.PU, vito^GD10890 has eye disc phenotype, suppressible | partially by bsk^DN.UAS/Scer\GAL4^ey.PU

(Marinho et al., 2011)

Scer\GAL4^ey.PU, vito^GD10890 has eye disc phenotype, suppressible by Hsap\NOL12^UAS.GFP, Scer\GAL4^ey.PU

(Marinho et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^ey.PU, vito^GD10890 has eye disc phenotype, non-suppressible by p53^5A-1-4/p53^5A-1-4

(Marinho et al., 2011)

Scer\GAL4^ey.PU, vito^GD10890 has eye disc phenotype, non-suppressible by p53^R155H.UAS.Ex/Scer\GAL4^ey.PU

(Marinho et al., 2011)

Other

Statement

Reference

Myc^UAS.cZa, Scer\GAL4^ptc-559.1, vito^GD10890 has nucleolus phenotype

(Marinho et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for Df(3L)H99 in the Scer\GAL4^ey.PU, vito^GD10890 background almost completely suppresses the increased apoptosis phenotype, but eye disc size is only partly rescued.

Homozygosity for p53^5A-1-4, or co-expression of p53^{R155H.Scer\UAS.Ex}, in the Scer\GAL4^ey.PU, vito^GD10890 background does not rescue the increased apoptosis phenotype or eye disc size.

Co-expression of bsk^DN.Scer\UAS in the Scer\GAL4^ey.PU, vito^GD10890 background partially suppresses the increased apoptosis phenotype, but eye disc size is not rescued.

Co-expression of vito^GD10890 reduces the size of Scer\GAL4^Act5C.PI-indcued dm^Scer\UAS.cZa clones to control levels. This is the consequence of partial reversion of the dm^Scer\UAS.cZa-induced cell size increase together with a decrease in the number of cells per clone.

Co-expression of vito^GD10890 considerably attenuates the increased cell size seen in Scer\GAL4^ptc-559.1, dm^Scer\UAS.cZa salivary glands. This is a result of significant reductions in cytoplasmic and nuclear volumes. The nucleoli display a segregated organization, with a peripheral granular region surrounding an internal fibrillar area.

(Marinho et al., 2011)

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\NOL12^{Scer\UAS.T:Avic\GFP} in the vito^GD10890, Scer\GAL4^ey.PU background fully rescues eye disc growth.

Coexpression of BacA\p35^Scer\UAS.cHa in the Scer\GAL4^ey.PU, vito^GD10890 background almost completely suppresses the increased apoptosis phenotype, but eye disc size is only partly rescued.

(Marinho et al., 2011)

Complementation and Rescue Data

Rescued by

vito^GD10890 is rescued by vito^UAS.cMa/Scer\GAL4^ey.PU

(Marinho et al., 2011)

Comments

Co-expression of vito^Scer\UAS.cMa in the vito^GD10890, Scer\GAL4^ey.PU background significantly rescues eye disc size, adult eye size and morphology, and several other cellular parameters.

(Marinho et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v34549

w¹¹¹⁸; P{GD10890}v34549

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts