FlyBase Allele Report: Dmel\ebd1[GD10952]

General Information

Symbol

Dmel\ebd1^GD10952

Species

D. melanogaster

Name

FlyBase ID

FBal0203741

Feature type

allele

Associated gene

Dmel\ebd1

Associated Insertion(s)

Carried in Construct

P{GD10952}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10952}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10952

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number, with Scer\GAL4^NP0001

(Tian et al., 2017)

increased occurrence of cell division, with Scer\GAL4^NP0001

(Tian et al., 2017)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

adult midgut epithelium, with Scer\GAL4^NP0001

(Tian et al., 2017)

enteroblast | adult stage | increased number, with Scer\GAL4^NP0001

(Tian et al., 2017)

intestinal stem cell | adult stage | increased number, with Scer\GAL4^NP0001

(Tian et al., 2017)

Detailed Description

Statement

Reference

Adult flies expressing ebd1^GD10952 under the control of Scer\GAL4^{Myo31DF-NP0001} (combined with Gal80[ts] to limit the time of expression to adulthood) display increased numbers of progenitor cells and intestinal stem cells that are grouped in chains or clusters as well as their increased mitotic activity.

(Tian et al., 2017)

Adults expressing ebd1^GD10952 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or semi-lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10952

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ebd1^GD10952, Scer\GAL4^NP7397 is a suppressor of increased cell number | adult stage phenotype of Apc^GD1333, Scer\GAL4^NP7397

(Tian et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

ebd1^GD10952, Scer\GAL4^NP7397 is a suppressor of adult midgut epithelium phenotype of Apc^GD1333, Scer\GAL4^NP7397

(Tian et al., 2017)

ebd1^GD10952, Scer\GAL4^NP7397 is a suppressor of intestinal stem cell | increased number | adult stage phenotype of Apc^GD1333, Scer\GAL4^NP7397

(Tian et al., 2017)

ebd1^GD10952, Scer\GAL4^NP7397 is a suppressor of enteroblast | increased number | adult stage phenotype of Apc^GD1333, Scer\GAL4^NP7397

(Tian et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of progenitor cells (intestinal stem cells and enteroblasts) observed in adult flies expressing Apc^GD1333 under the control of Scer\GAL4^esg-NP7397 is fully suppressed by co-expression of ebd1^GD10952.

(Tian et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v26180

w¹¹¹⁸; P{GD10952}v26180

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10952

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG3371^GD10952

ebd1^GD10952

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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