Males expressing natalisin^GD5691 under the control of Scer\GAL4^n-syb.PS (in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi efficiency) show strongly reduced mating frequency, with only approximately 10% succeeding in copulation of wild-type females in an hour long assay, compared to a success rate of 40-80% for wild-type males mated to wild-type females. The mutant males show an increased courtship initiation latency.

Females expressing natalisin^GD5691 under the control of Scer\GAL4^n-syb.PS (in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi efficiency) do not succeed in mating when paired with wild-type males for 1 hour. These females do not actively reject courting males, but instead show significantly elevated levels of grooming, which likely prevent the males from being able to access the females for attempted copulation.

Expression under the control of Scer\GAL4^Mef2.PR results in early pupal lethality.

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage.