FlyBase Allele Report: Dmel\Gdap1[GD10713]

General Information

Symbol

Dmel\Gdap1^GD10713

Species

D. melanogaster

Name

FlyBase ID

FBal0204338

Feature type

allele

Associated gene

Dmel\Gdap1

Associated Insertion(s)

Carried in Construct

P{GD10713}

Also Known As

UAS-Gdap1^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10713}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10713

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Charcot-Marie-Tooth disease

CEA

(López Del Amo et al., 2017, López Del Amo et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | progressive, with Scer\GAL4^GMR.PU

(López Del Amo et al., 2015)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^Mhc.PU

(López Del Amo et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Gdap1^GD10713 under the control of Scer\GAL4^GMR.PU results in age-dependent loss of photoreceptors, which is higher than that seen in control flies at both 1 week and 5 weeks of age. 1 week old flies show a loss of the rhabdomere terminal web region compared to controls and show rhabdomere degeneration and 5 week old flies have large, elongated and misplaced mitochondria next to the rhabdomere. The lamina cartridge is reduced in size, has abnormal morphology and has a smaller axonal profile than normal in both 1 week and 5 week old flies. The average area of the capitate projections is larger than normal in 1 week old flies.

Old flies expressing Gdap1^GD10713 under the control of Scer\GAL4^Mhc.PU often have a held-up wing phenotype.

1 week old flies expressing Gdap1^GD10713 under the control of Scer\GAL4^Mhc.PU show degeneration of the longitudinal indirect flight muscles and there is an accumulation of partially fused mitochondria. At 5 weeks of age, there is more severe muscle degeneration and extremely large and elongated mitochondria are seen.

(López Del Amo et al., 2015)

Adults expressing Gdap1^GD10713 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10713

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Gdap1^GD10713, Scer\GAL4^GMR.PU has abnormal neuroanatomy | adult stage | progressive phenotype, suppressible | partially by Hsap\GDAP1^UAS.ECFP, Scer\GAL4^GMR.PU

(López Del Amo et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Gdap1^GD10713, Scer\GAL4^GMR.PU has eye photoreceptor cell | progressive phenotype, suppressible | partially by Hsap\GDAP1^UAS.ECFP, Scer\GAL4^GMR.PU

(López Del Amo et al., 2015)

Gdap1^GD10713, Scer\GAL4^GMR.PU has mitochondrion phenotype, suppressible | partially by Hsap\GDAP1^UAS.ECFP, Scer\GAL4^GMR.PU

(López Del Amo et al., 2015)

Gdap1^GD10713, Scer\GAL4^GMR.PU has rhabdomere phenotype, suppressible | partially by Hsap\GDAP1^UAS.ECFP, Scer\GAL4^GMR.PU

(López Del Amo et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Hsap\GDAP1^{Scer\UAS.T:Avic\GFP-ECFP} partially rescues the age-dependent loss of photoreceptors, rhabdomere degeneration and mitochondrial defects seen in flies expressing Gdap1^GD10713 under the control of Scer\GAL4^GMR.PU.

(López Del Amo et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v21624

w¹¹¹⁸; P{GD10713}v21624

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10713

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG4623^GD10713

Gdap1^GD10713

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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