FlyBase Allele Report: Dmel\Usp12-46[GD12106]

General Information

Symbol

Dmel\Usp12-46^GD12106

Species

D. melanogaster

Name

FlyBase ID

FBal0205291

Feature type

allele

Associated gene

Dmel\Usp12-46

Associated Insertion(s)

Carried in Construct

P{GD12106}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12106}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12106

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

eo sensory structure, with Scer\GAL4^pnr-MD237

(Moretti et al., 2012)

mesonotum, with Scer\GAL4^pnr-MD237

(Moretti et al., 2012)

tormogen cell, with Scer\GAL4^pnr-MD237

(Moretti et al., 2012)

Detailed Description

Statement

Reference

Scer\GAL4^pnr-MD237-mediated expression of Usp12-46^GD12106 disorganizes the bristle socket pattern on the thoracic surface of adult flies. The overall shape and aspect of the notum is significantly affected; loss of bristles and duplication of sockets is frequent.

(Moretti et al., 2012)

Expression of Usp12-46^GD12106 under the control of Scer\GAL4^bbg-C96 does not result in a wing phenotype.

Expression of Usp12-46^GD12106 under the control of Scer\GAL4^dpp.PU does not result in a bristle phenotype.

(Zhang et al., 2012)

Adults expressing Usp12-46^GD12106 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of Usp12-46^GD12106 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD12106} insertion line used.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12106

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^pnr-MD237, Usp12-46^GD12106 has tormogen cell phenotype, suppressible by N[+]/N^55e11

(Moretti et al., 2012)

Suppressor of

Statement

Reference

Scer\GAL4^hh-Gal4/Usp12-46^GD12106 is a suppressor of wing margin bristle | ectopic phenotype of Scer\GAL4^hh-Gal4, arr^{UAS.cPa.Tag:HA}

(Spencer et al., 2023)

Scer\GAL4^hh-Gal4/Usp12-46^GD12106 is a suppressor of wing blade phenotype of Scer\GAL4^hh-Gal4, arr^{UAS.cPa.Tag:HA}

(Spencer et al., 2023)

Scer\GAL4^bbg-C96/Usp12-46^GD12106 is a suppressor of wing margin bristle | ectopic phenotype of Scer\GAL4^bbg-C96, arr^{UAS.cPa.Tag:HA}

(Spencer et al., 2023)

Scer\GAL4^bbg-C96/Usp12-46^GD12106 is a suppressor of wing blade phenotype of Scer\GAL4^bbg-C96, arr^{UAS.cPa.Tag:HA}

(Spencer et al., 2023)

Other

Statement

Reference

Scer\GAL4^pnr-MD237, Usp12-46^GD12106, spdo^KK103906 has tormogen cell phenotype

(Moretti et al., 2012)

Scer\GAL4^pnr-MD237, Usp12-46^GD12106, spdo^KK103906 has mesonotum phenotype

(Moretti et al., 2012)

Scer\GAL4^pnr-MD237, Usp12-46^GD12106, spdo^KK103906 has eo sensory structure phenotype

(Moretti et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Introducing N^55e11/+ into the Scer\GAL4^pnr-MD237, Usp12-46^GD12106 background results in a partial restoration of normal phenotype because the cell fate transformation into socket cells is seldom observed, although lateral inhibition problems as well as malformation of the notum are still obvious.

When both spdo^KK103906 and Usp12-46^GD12106 are expressed using Scer\GAL4^pnr-MD237, an intermediate phenotype is observed characterized by the production of double-bristles or even double-sockets.

(Moretti et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v27799

w¹¹¹⁸; P{GD12106}v27799

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12106

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG7023^GD12106

Usp12-46^GD12106

Usp46ⁱ¹

(Spencer et al., 2023)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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