FlyBase Allele Report: Dmel\CCT2[GD5201]

General Information

Symbol

Dmel\CCT2^GD5201

Species

D. melanogaster

Name

FlyBase ID

FBal0205300

Feature type

allele

Associated gene

Dmel\CCT2

Associated Insertion(s)

Carried in Construct

P{GD5201}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD5201}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD5201

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

larval neuroblast | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

neuroblast | larval stage, with Scer\GAL4^wor.PA

(Kuang et al., 2014)

Detailed Description

Statement

Reference

Expression of CG7033^GD5201 under the control of Scer\GAL4^wor.PA induces neuroblast loss in larval brains. Many of the remaining neuroblasts exhibit necrosis markers, including aggregation of mitochondria, elevated levels of calcium ions and the presence of ubiquitin-conjugated protein aggregates.

(Kuang et al., 2014)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in loss of neuroblasts in the larval brain without any change in size of the remaining neuroblasts.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in mild defects in the shape of neuroblasts in the larval brain.

(Neumüller et al., 2011)

Adults expressing CG7033^GD5201 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in early larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the larval body wall muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5201

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

CCT2^GD5201, Scer\GAL4^wor.PA has abnormal neuroanatomy | larval stage phenotype, suppressible | partially by fzy^UAS.cKa, Scer\GAL4^wor.PA

(Kuang et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

CCT2^GD5201, Scer\GAL4^wor.PA has neuroblast | larval stage phenotype, suppressible | partially by fzy^UAS.cKa, Scer\GAL4^wor.PA

(Kuang et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of fzy^Scer\UAS.cKa suppresses the neuroblast loss seen in larval brains expressing CG7033^GD5201 under the control of Scer\GAL4^wor.PA. However, these surviving neuroblasts display phenotypes indicative of mitotic catastrophe including multinucleation and enlargement of cell diameter.

(Kuang et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v41190