FlyBase Allele Report: Dmel\NdufAF1[GD6338]

General Information

Symbol

Dmel\NdufAF1^GD6338

Species

D. melanogaster

Name

FlyBase ID

FBal0205530

Feature type

allele

Associated gene

Dmel\NdufAF1

Associated Insertion(s)

Carried in Construct

P{GD6338}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD6338}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD6338

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of mitochondrial metabolism disease

CEA

(Cho et al., 2012)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of mitochondrial metabolism disease

is ameliorated by Scer\NDI1^UAS.cBa

(Cho et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal heat stress response, with Scer\GAL4^da.PU

(Cho et al., 2012)

abnormal starvation stress response, with Scer\GAL4^da.PU

(Cho et al., 2012)

decreased body size | pupal stage, with Scer\GAL4^da.PU

(Cho et al., 2012)

flightless, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

partially lethal - majority die, with Scer\GAL4^da.PU

(Cho et al., 2012)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

mitochondrion, with Scer\GAL4^da.PU

(Cho et al., 2012)

myofibril, with Scer\GAL4^da.PU

(Cho et al., 2012)

Detailed Description

Statement

Reference

Pupae expressing CIA30^GD6338 via Scer\GAL4^da.PU are small compared to controls. Adult flies show an ~30% reduction in body weight compared to controls and show severe degeneration of both myofibrils and mitochondria in thoracic flight muscles.

Flies expressing CIA30^GD6338 via Scer\GAL4^da.PU show: a drastically reduced ability to recover from exposure to hypoxia; increased sensitivity to hyperoxia, greater susceptibility to wet starvation; and drastically reduced resistance to elevated temperature.

(Cho et al., 2012)

Adults expressing CIA30^GD6338 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in flightless flies or adult/pharate lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in morphologically wild-type myofibrils in the adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the adult indirect flight muscles.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

6338

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

NdufAF1^GD6338, Scer\GAL4^da.PU has partially lethal - majority die phenotype, suppressible by Scer\NDI1^UAS.cBa, Scer\GAL4^da.PU

(Cho et al., 2012)

NdufAF1^GD6338, Scer\GAL4^da.PU has decreased body size | pupal stage phenotype, suppressible by Scer\NDI1^UAS.cBa, Scer\GAL4^da.PU

(Cho et al., 2012)

NdufAF1^GD6338, Scer\GAL4^da.PU has abnormal starvation stress response phenotype, suppressible | partially by Scer\NDI1^UAS.cBa, Scer\GAL4^da.PU

(Cho et al., 2012)

NdufAF1^GD6338, Scer\GAL4^da.PU has abnormal heat stress response phenotype, suppressible | partially by Scer\NDI1^UAS.cBa, Scer\GAL4^da.PU

(Cho et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

NdufAF1^GD6338, Scer\GAL4^da.PU has mitochondrion phenotype, suppressible | partially by Scer\NDI1^UAS.cBa, Scer\GAL4^da.PU

(Cho et al., 2012)

NdufAF1^GD6338, Scer\GAL4^da.PU has myofibril phenotype, suppressible by Scer\NDI1^UAS.cBa, Scer\GAL4^da.PU

(Cho et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Scer\NDI1^Scer\UAS.cBa rescues the sensitivity to hypoxia and hyperoxia, and partially restores normal survival upon wet starvation and heat stress in flies expressing CIA30^GD6338 via Scer\GAL4^da.PU.

(Cho et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v14859

w¹¹¹⁸; P{GD6338}v14859

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

6338

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG7598^GD6338

CIA30^GD6338

NdufAF1^GD6338

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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