Expression of Scer\NDI1Scer\UAS.cBa under the control of Scer\GAL4da.G32 does not affect the expression or assembly of the endogenous respiratory chain machinery. In contrast to control flies, mitochondria from Scer\NDI1Scer\UAS.cBa-expressing flies support a substantial rotenone-insensitive substrate oxidation. Expression of Scer\NDI1Scer\UAS.cBa in fly mitochondria leads to a 30% increase in glutamate/malate-stimulated (complex-I-specific) respiration, while complex II (succinate-stimulated)-specific respiration is unaffected by Scer\NDI1Scer\UAS.cBa expression. These flies also exhibit a 28% increse in NADH-ubiquinone oxidoreductase (Complex I) enzymatic activity and a 2.6 fold increase in rotenone-insensitive NADH-ubiquinone oxidoreductase activity. Mitochondrial reactive oxygen species production is unaffected. Expression of Scer\NDI1Scer\UAS.cBa under the control of Scer\GAL4da.G32 confers a 24% increase in ATP levels and a 16% increase in metabolic rate compared to controls.
Ubiquitous induction of Scer\NDI1Scer\UAS.cBa during both development and adulthood using Scer\GAL4αTub84B.Switch.PK has no robust positive effects on longevity in male or female flies.
Scer\NDI1Scer\UAS.cBa expression in adipose tissue under the control of Scer\GAL4Switch1.106 results in shortened lifespan in both male and female flies.
Neuronal expression of Scer\NDI1Scer\UAS.cBa under the control of Scer\GAL4elav.Switch.PO increases lifespan by up to 21% in females and by up to 7% in males. This neuronal expression under the control of Scer\GAL4elav.Switch.PO leads to a 16% increase in complex I enzymatic activity in mitochondria isolated from heads and confers a 65% increase in rotenone-insensitive NADH-ubiquinone oxidoreductase activity and 28% increase in ATP levels in head tissue. This neuronal expression does not lead to an increase in CO[] production in living flies, confers a 51% decrease in the level of reactive oxygen species in the brains of 45-day-old flies compared to non-induced controls. Finally, this neuronal expression results in an improved tolerance to rotenone and paraquat poisoning but does not improve their ability to withstand starvation.
RU486-induced expression of Scer\NDI1Scer\UAS.cBa in fly neurons (under the control of Scer\GAL4elav.Switch.PO does not decrease fertility relative to uninduced cohorts and has no effect on physical activity levels.
Expression of Scer\NDI1Scer\UAS.cBa in muscle cells under the control of Scer\GAL4Mhc.PU has no major impact on longevity in male or female flies.