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General Information
Symbol
Scer\NDI1UAS.cBa
Species
S. cerevisiae
Name
Saccharomyces cerevisiae UAS construct a of Bahadorani
FlyBase ID
FBal0258475
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Ndi1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of Scer\NDI1 coding sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Scer\NDI1Scer\UAS.cBa under the control of Scer\GAL4da.G32 does not affect the expression or assembly of the endogenous respiratory chain machinery. In contrast to control flies, mitochondria from Scer\NDI1Scer\UAS.cBa-expressing flies support a substantial rotenone-insensitive substrate oxidation. Expression of Scer\NDI1Scer\UAS.cBa in fly mitochondria leads to a 30% increase in glutamate/malate-stimulated (complex-I-specific) respiration, while complex II (succinate-stimulated)-specific respiration is unaffected by Scer\NDI1Scer\UAS.cBa expression. These flies also exhibit a 28% increse in NADH-ubiquinone oxidoreductase (Complex I) enzymatic activity and a 2.6 fold increase in rotenone-insensitive NADH-ubiquinone oxidoreductase activity. Mitochondrial reactive oxygen species production is unaffected. Expression of Scer\NDI1Scer\UAS.cBa under the control of Scer\GAL4da.G32 confers a 24% increase in ATP levels and a 16% increase in metabolic rate compared to controls.

Ubiquitous induction of Scer\NDI1Scer\UAS.cBa during both development and adulthood using Scer\GAL4╬▒Tub84B.Switch.PK has no robust positive effects on longevity in male or female flies.

Scer\NDI1Scer\UAS.cBa expression in adipose tissue under the control of Scer\GAL4Switch1.106 results in shortened lifespan in both male and female flies.

Neuronal expression of Scer\NDI1Scer\UAS.cBa under the control of Scer\GAL4elav.Switch.PO increases lifespan by up to 21% in females and by up to 7% in males. This neuronal expression under the control of Scer\GAL4elav.Switch.PO leads to a 16% increase in complex I enzymatic activity in mitochondria isolated from heads and confers a 65% increase in rotenone-insensitive NADH-ubiquinone oxidoreductase activity and 28% increase in ATP levels in head tissue. This neuronal expression does not lead to an increase in CO[[2]] production in living flies, confers a 51% decrease in the level of reactive oxygen species in the brains of 45-day-old flies compared to non-induced controls. Finally, this neuronal expression results in an improved tolerance to rotenone and paraquat poisoning but does not improve their ability to withstand starvation.

RU486-induced expression of Scer\NDI1Scer\UAS.cBa in fly neurons (under the control of Scer\GAL4elav.Switch.PO does not decrease fertility relative to uninduced cohorts and has no effect on physical activity levels.

Expression of Scer\NDI1Scer\UAS.cBa in muscle cells under the control of Scer\GAL4Mhc.PU has no major impact on longevity in male or female flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

Co-expression of Scer\NDI1Scer\UAS.cBa ameliorates the reduction in locomotor activity seen in adults expressing Hsap\SOD1D83S.Scer\UAS under the control of Scer\GAL4elav.PU.

Co-expression of Scer\NDI1Scer\UAS.cBa rescues the sensitivity to hypoxia and hyperoxia, and partially restores normal survival upon wet starvation and heat stress in flies expressing CIA30GD6338 via Scer\GAL4da.PU.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Scer\NDI1Scer\UAS.cBa
Scer\NDI1UAS.cBa
Scer\Ndi1Scer\UAS.cBa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Bahadorani
Secondary FlyBase IDs
    References (4)