UASt regulatory sequences drive expression of an inverted repeat.
Expressing C1GalTAGD1019 under the control of Scer\GAL4srp.Hemo results in significantly fewer macrophages within the germ band and significantly more macrophages on the yolk of stage 12 embryos, as compared to controls; the sum of the macrophages in the yolk and germband is the same in the controls; there are no changes in the number of macrophages on (i.e. migrating along) the ventral nerve cord.
Adults expressing C1GalTAGD1019 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Expression of C1GalTAGD1019 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or complete lethality, depending on the particular P{GD1019} insertion line used.
Expression under the control of Scer\GAL4Mef2.PR results in late pupal lethality.
Expression under the control of Scer\GAL4pnr-MD237 may result in semi-lethality, depending on the insertion line used.