FlyBase Allele Report: Dmel\SERCA[GD436]

General Information

Symbol

Dmel\SERCA^GD436

Species

D. melanogaster

Name

FlyBase ID

FBal0206475

Feature type

allele

Associated gene

Dmel\SERCA

Associated Insertion(s)

Carried in Construct

P{GD436}

Also Known As

UAS-SERCA-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD436}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD436

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult fat body, with Scer\GAL4^ppl.PP

(Bi et al., 2014)

lipid droplet, with Scer\GAL4^ppl.PP

(Bi et al., 2014)

wing, with Scer\GAL4^elav.PLu

(Dietzl et al., 2007)

Detailed Description

Statement

Reference

Expression of Ca-P60A^GD436 under the control of Scer\GAL4^ppl.PP results in smaller lipid droplets in fat cells compared to controls. The relative glyceride level is significantly decreased ro only ~50% of the wild type level.

(Bi et al., 2014)

Adults expressing SERCA^GD436 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in early pupal lethality.

(Schnorrer et al., 2010)

Expression of Ca-P60A^GD436 under the control of Scer\GAL4^elav.PLu results in flies with abnormal wing posture, defective flight behavior and mild locomotion defects.

(Dietzl et al., 2007)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

SERCA^GD436, Scer\GAL4^ppl.PP has adult fat body phenotype, enhanceable by Seipin^GD3208, Scer\GAL4^ppl.PP

(Bi et al., 2014)

SERCA^GD436, Scer\GAL4^ppl.PP has lipid droplet phenotype, enhanceable by Seipin^GD3208, Scer\GAL4^ppl.PP

(Bi et al., 2014)

NOT suppressed by

Statement

Reference

SERCA^GD436, Scer\GAL4^ppl.PP has adult fat body phenotype, non-suppressible by Seipin^UAS.EYFP, Scer\GAL4^ppl.PP

(Bi et al., 2014)

SERCA^GD436, Scer\GAL4^ppl.PP has lipid droplet phenotype, non-suppressible by Seipin^UAS.EYFP, Scer\GAL4^ppl.PP

(Bi et al., 2014)

Enhancer of

Statement

Reference

SERCA^GD436, Scer\GAL4^ppl.PP is an enhancer of adult fat body phenotype of Scer\GAL4^ppl.PP, Seipin^GD3208

(Bi et al., 2014)

SERCA^GD436, Scer\GAL4^ppl.PP is an enhancer of lipid droplet phenotype of Scer\GAL4^ppl.PP, Seipin^GD3208

(Bi et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Seipin^GD3208 and Ca-P60A^GD436 under the control of Scer\GAL4^ppl.PP further enhances the reduction in lipid droplet size and decrease in glyceride levels seen when either transgene is expressed alone.

Expression of Seipin^{Scer\UAS.T:Avic\GFP-EYFP} does not rescue the reduction in lipid droplet size seen in fat body cells when Ca-P60A^GD436 is expressed under the control of Scer\GAL4^ppl.PP.

(Bi et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v4474

w¹¹¹⁸; P{GD436}v4474

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

ATP2A1^kum

(Iyer et al., 2018)

Ca-P60A^GD436

SERCA^GD436

SERCA^RNAi#1

(Weiss et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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