FlyBase Allele Report: Dmel\Cat[GD1291]

General Information

Symbol

Dmel\Cat^GD1291

Species

D. melanogaster

Name

FlyBase ID

FBal0206520

Feature type

allele

Associated gene

Dmel\Cat

Associated Insertion(s)

Carried in Construct

P{GD1291}

Also Known As

UAS-Cat-IR, UAS-catalase^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1291}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1291

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult heart, with Scer\GAL4^Ugt36A1.PK

(Lim et al., 2014)

adult heart | progressive | RU486 conditional, with Scer\GAL4^{Hand.ΔVM.Switch}

(Monnier et al., 2012)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Elevation of reactive oxygen species levels in nonmyocytic pericardial cells, through expression of Cat^GD1291 under the control of Scer\GAL4^Dot.PK results in an abnormal heart rhythm, with an increased frequency of irregular beats, a broader distribution of HPs and significantly increased the arrhythmia index. There is significant cardiac narrowing, with significant reductions in both the diastolic and systolic diameters of young and old flies compared with control flies.

Expression of Cat^GD1291 in cardiomyocytes and nonmyocytic pericardial cells, under the control of Scer\GAL4^Hand.PA, which results in a significant increase in the level of physiological reactive oxygen species, has no significant effects on any major aspects of heart function, including heart rhythm or heart tube dimensions.

(Lim et al., 2014)

Expression via P{GAL4-tim.E}27 (in the presence of Dcr-2^Scer\UAS.cDa) results in a reduction in the 'rhythmic strength' value of flies classified as rhythmic and a prolonged period.

(Mandilaras and Missirlis, 2012)

Expression of Cat^GD1291 under the control of Scer\GAL4^{Hand.ΔVM.Switch} (initiated by feeding flies RU486 during adulthood) results in strong age-dependent heart defects. The heart period (mean time between successive end-diastolic positions) is higher in 60 day old mutant flies compared to age-matched controls.

(Monnier et al., 2012)

Adults expressing Cat^GD1291 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in morphologically wild-type myofibrils in the adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in wild-type sarcomeres in the adult indirect flight muscles.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 10-20% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 30-40% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1291

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Cat^GD1291, Scer\GAL4^Ugt36A1.PK has abnormal oxidative stress response phenotype, suppressible by p38b^UAS.ORF, Scer\GAL4^Ugt36A1.PK

(Lim et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Cat^GD1291, Scer\GAL4^Ugt36A1.PK has adult heart phenotype, suppressible by p38b^UAS.ORF, Scer\GAL4^Ugt36A1.PK

(Lim et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of p38b^Scer\UAS.ORF significantly suppresses the cardiac defects associated with expression of Cat^GD1291 under the control of Scer\GAL4^Dot.PK.

(Lim et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v6283

w¹¹¹⁸; P{GD1291}v6283

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1291

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Cat^GD1291

Cat^RNAi

(Tamamouna et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

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