FlyBase Allele Report: Dmel\Ctr1B[GD2859]

General Information

Symbol

Dmel\Ctr1B^GD2859

Species

D. melanogaster

Name

FlyBase ID

FBal0206702

Feature type

allele

Associated gene

Dmel\Ctr1B

Associated Insertion(s)

Carried in Construct

P{GD2859}

Also Known As

Ctr1B^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD2859}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD2859

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

some die during larval stage, with Scer\GAL4^elav-C155

(Mercer et al., 2016)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Ctr1B^GD2859 driven in the thoracic midline by Scer\GAL4^pnr-MD237 does not cause significant observable effects.

(Mercer and Burke, 2016)

Expression of Ctr1B^GD2859 driven by Scer\GAL4^elav-C155 significantly increases larval lethality compared to controls.

(Mercer et al., 2016)

Expression of Ctr1B^GD2859 under the control of Scer\GAL4^pnr-MD237 has no effect on pigmentation in the adult dorsal cuticle of the thorax and abdomen (the domain of Scer\GAL4 expression).

Expression of Ctr1B^GD2859 under the control of Scer\GAL4^GMR.PFa has no effect on the eye.

(Binks et al., 2010)

Adults expressing Ctr1B^GD2859 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

2859

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Ctr1B^GD2859, Scer\GAL4^pnr-MD237 is an enhancer of visible phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

Ctr1B^GD2859, Scer\GAL4^pnr-MD237 is an enhancer of abnormal body color | adult stage phenotype of Ctr1A^GD16726, Scer\GAL4^pnr-MD237

(Binks et al., 2010)

NOT Enhancer of

Statement

Reference

Ctr1B^GD2859, Scer\GAL4^elav-C155 is a non-enhancer of majority die during larval stage phenotype of Gclc^GD9767, Scer\GAL4^elav-C155

(Mercer et al., 2016)

Phenotype Manifest In

Enhancer of

Statement

Reference

Ctr1B^GD2859, Scer\GAL4^pnr-MD237 is an enhancer of adult thorax phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

Ctr1B^GD2859, Scer\GAL4^pnr-MD237 is an enhancer of scutellum phenotype of Grx1^GD10587, Scer\GAL4^pnr-MD237

(Mercer and Burke, 2016)

Ctr1B^GD2859, Scer\GAL4^pnr-MD237 is an enhancer of adult cuticle phenotype of Ctr1A^GD16726, Scer\GAL4^pnr-MD237

(Binks et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Ctr1B^GD2859 enhances the thoracic cleft and reduced scutellum seen in flies with expression of Grx1^GD10587 driven by Scer\GAL4^pnr-MD237.

(Mercer and Burke, 2016)

Co-expression of Ctr1B^GD2859 does not significantly enhance larval lethality seen with expression of Gclc^GD9767 driven by Scer\GAL4^elav-C155.

(Mercer et al., 2016)

Co-expression of Ctr1B^GD2859 enhances the cuticle hypopigmentation phenotype caused by expression of a weak Ctr1A^GD16726 line under the control of Scer\GAL4^pnr-MD237.

(Binks et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v5804