FlyBase Allele Report: Dmel\Doa[GD8588]

General Information

Symbol

Dmel\Doa^GD8588

Species

D. melanogaster

Name

FlyBase ID

FBal0206880

Feature type

allele

Associated gene

Dmel\Doa

Associated Insertion(s)

Carried in Construct

P{GD8588}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD8588}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD8588

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Voßfeldt et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal starvation stress response | larval stage | somatic clone, with Scer\GAL4^Act.PU

(Tang et al., 2018)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

Phenotype Manifest In

autophagosome | larval stage | somatic clone | conditional, with Scer\GAL4^Act.PU

(Tang et al., 2018)

embryonic/larval fat body | larval stage | somatic clone | conditional, with Scer\GAL4^Act.PU

(Tang et al., 2018)

Detailed Description

Statement

Reference

Larval fat body clones expressing Doa^GD8588 under the control of Scer\GAL4^Act.PU show impaired induction of autophagosomes (identified as mCherry-Atg8 puncta) in response to starvation.

(Tang et al., 2018)

Expression of Doa^GD8588 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver results in viable flies or partial lethality, depending on the particular P{GD8588} insertion line used.

(Neely et al., 2010)

Adults expressing Doa^GD8588 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in adult/pharate lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development