FlyBase Allele Report: Dmel\Ent1[GD783]

General Information

Symbol

Dmel\Ent1^GD783

Species

D. melanogaster

Name

FlyBase ID

FBal0206972

Feature type

allele

Associated gene

Dmel\Ent1

Associated Insertion(s)

Carried in Construct

P{GD783}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD783}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD783

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | pharate adult stage, with Scer\GAL4^Act5C.PI

(Cliffe et al., 2009)

lethal | pupal stage, with Scer\GAL4^Act5C.PI

(Cliffe et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^Bx-MS1096

(Cliffe et al., 2009)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

scutellar bristle, with Scer\GAL4^Act5C.PI

(Cliffe et al., 2009)

scutellar bristle | ectopic, with Scer\GAL4^Act5C.PI

(Cliffe et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^Bx-MS1096

(Cliffe et al., 2009)

Detailed Description

Statement

Reference

Adults expressing Ent1^GD783 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Adult escapers expressing Ent1^GD783 under the control of Scer\GAL4^Act5C.PI at 25[o]C show two scutellar bristle phenotypes; abnormally short, thick bristles as well as ectopic bristles are seen.

Expression of Ent1^GD783 under the control of Scer\GAL4^Bx-MS1096 results in an up-curled wing phenotype in 93.5% of flies.

(Cliffe et al., 2009)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 80-90% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Ent1^GD783, Pi3K92E^A2860C.UAS, Scer\GAL4^Bx-MS1096 has visible phenotype

(Cliffe et al., 2009)

Phenotype Manifest In

Other

Statement

Reference

Ent1^GD783, Pi3K92E^A2860C.UAS, Scer\GAL4^Bx-MS1096 has wing phenotype

(Cliffe et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

100% of flies co-expressing Pi3K92E^{A2860C.Scer\UAS} and Ent1^GD783 under the control of Scer\GAL4^Bx-MS1096 have a strongly up-curled phenotype.

(Cliffe et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Ent1^GD783 is rescued by Scer\GAL4^Act5C.PI/Ent1^EP2066

(Cliffe et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v49328

w¹¹¹⁸ P{GD783}v49328

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Ent1^GD783

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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