Expressing Fer2LCH^GD6348 under the control of Scer\GAL4^NP3084 is lethal in a major proportion of flies fed a regular diet, more extreme in flies fed an iron-depleted diet and only in a minor proportion of flies fed an iron-rich diet.

Expression via P{GAL4-tim.E}27 (in the presence of Dcr-2^Scer\UAS.cDa) results in robust reduction in the the number of flies showing rhythmic behaviour in constant darkness, a concomitant reduction in the 'rhythmic strength' value of flies classified as rhythmic, and a prolonged period.

Under 12:12 hour alternating light/dark cycles, control and P{GAL4-tim.E}27 Fer2LCH^GD6348 Dcr-2^Scer\UAS.cDa flies show no apparent differences in locomotor circadian rhythm. Rhythm breaks down under constant darkness conditions, and is restored upon re-establishment of light/dark cycles. Clock neurons are not killed by Fer2LCH^GD6348 expression.

Adults expressing Fer2LCH^GD6348 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Fer2LCH^GD6348, Scer\GAL4^NP3084 has partially lethal - majority die phenotype, non-suppressible by Tsf1^NIG.6186R, Scer\GAL4^NP3084

Fer2LCH^GD6348, Scer\GAL4^NP3084 has partially lethal - majority die | nutrition conditional phenotype, non-suppressible by Tsf1^NIG.6186R, Scer\GAL4^NP3084

Fer2LCH^GD6348, Scer\GAL4^NP3084 has partially lethal - majority live | nutrition conditional phenotype, non-suppressible by Tsf1^NIG.6186R, Scer\GAL4^NP3084