FlyBase Allele Report: Dmel\HP1e[GD13814]

General Information

Symbol

Dmel\HP1e^GD13814

Species

D. melanogaster

Name

FlyBase ID

FBal0207269

Feature type

allele

Associated gene

Dmel\HP1e

Associated Insertion(s)

Carried in Construct

P{GD13814}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD13814}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD13814

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryo | embryonic stage 1 | paternal effect

(Levine et al., 2015)

Detailed Description

Statement

Reference

Males expressing HP1e^GD13814 under the control of Scer\GAL4^Act5C.PI produce abundant motile sperm that transfer to females, fertilize eggs and initiate embryogenesis.

The embryos produced by crossing males expressing HP1e^GD13814 under the control of Scer\GAL4^Act5C.PI with wild type mothers (referred to as Paternal Effect Lethal (PEL) embryos) arrest after only a few rounds of zygotic mitosis. Aged embryos exhibit increasingly asynchronous nuclear cycling and acute mitotic catastrophe. Condensation of the male and female chromosomes is asynchronous: the paternal chromosome is less condensed than the maternal genome. The embryos do not exhibit any gross defects in maternal chromatin dynamics, and exhibit stereotypical centrosome and spindle morphology. As in wild type, sperm DNA undergoes the protamine-to-histone transition, decondenses, migrates towards the maternal pronucleus and enters into the first mitosis. However, at metaphase the paternal DNA fails to condense synchronously with the maternal chromosomes and fails to separate on the mitotic spindle. A prominent chromatin bridge appears at the first anaphase following this asynchronous metaphase.

The telophase chromatin bridge seen in embryos fathered by flies expressing HP1e^GD13814 under the control of Scer\GAL4^Act5C.PI is contains the Y chromosome in 94% of male embryos. The maternal X is never found in the bridge. In female embryos only the paternal X chromosome is trapped as a bridge. Chromosomes 2 and 3 are trapped at low levels in both male and female embryos.

(Levine et al., 2015)

Adults expressing HP1e^GD13814 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of HP1e^GD13814 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD13814} insertion line used.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

13814

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

HP1e^GD13814 has abnormal mitotic cell cycle | paternal effect | embryonic stage 1 phenotype, non-suppressible by Hira^185b

(Levine et al., 2015)

NOT Suppressor of

Statement

Reference

HP1e^GD13814/Scer\GAL4^Act5C.PI is a non-suppressor of abnormal mitotic cell cycle | embryonic stage 1 phenotype of Hira^185b

(Levine et al., 2015)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

HP1e^GD13814 has embryo | embryonic stage 1 phenotype, non-suppressible by Hira^185b

(Levine et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Crossing to homozygous Hira^185b mutant females suppresses the early mitotic arrest phenotype observed in embryos produced when males expressing HP1e^GD13814 under the control of Scer\GAL4^Act5C.PI are crossed to wild type females. As in homozygous Hira^185b mutants alone, the maternal DNA continues to cycle until late embryogenesis.

(Levine et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

HP1e^GD13814 is rescued by HP1e^rescue/Scer\GAL4^Act5C.PI

(Levine et al., 2015)

Comments

Expression of HP1e^rescue rescues the male sterility seen when HP1e^GD13814 is expressed under the control of Scer\GAL4^Act5C.PI.

(Levine et al., 2015)

Images (0)