FlyBase Allele Report: Dmel\Mical[GD9629]

General Information

Symbol

Dmel\Mical^GD9629

Species

D. melanogaster

Name

FlyBase ID

FBal0207574

Feature type

allele

Associated gene

Dmel\Mical

Associated Insertion(s)

Carried in Construct

P{GD9629}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD9629}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD9629

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates tauopathy

modeled by Hsap\MAPT^UAS.0N3R.cFa

(Prifti et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

flightless, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010, Beuchle et al., 2007)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^elav-C155

(Prifti et al., 2022)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

flight muscle cell, with Scer\GAL4^Mef2.PR

(Langer et al., 2010)

indirect flight muscle cell, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010, Beuchle et al., 2007)

larval somatic muscle cell, with Scer\GAL4^Mef2.PR

(Beuchle et al., 2007)

myofibril, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

sarcomere, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010, Beuchle et al., 2007)

Detailed Description

Statement

Reference

Animals expressing Mical^GD9629 under the control of Scer\GAL4^Mef2.PR have irregular flight muscle myofibrils.

(Langer et al., 2010)

Adults expressing Mical^GD9629 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in irregular adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in irregular myofibrils in the adult indirect flight muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in missing sarcomeres in the adult indirect flight muscles.

(Schnorrer et al., 2010)

Expression of Mical^GD9629 under the control of Scer\GAL4^OK371 does not result in any obvious defects in the organisation of myosin filaments in either larval muscles or in adult indirect flight muscles.

Expression of Mical^GD9629 under the control of Scer\GAL4^Mef2.PR results in a mutant myosin filament phenotype, with sarcomeric patterning being disrupted; myosin filaments form needle-like aggregates in muscles of third instar larvae and a felt-like network in indirect flight muscles of adult flies. The flies show a completely flightless phenotype.

(Beuchle et al., 2007)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development