FlyBase Allele Report: Dmel\Mekk1[GD9945]

General Information

Symbol

Dmel\Mekk1^GD9945

Species

D. melanogaster

Name

FlyBase ID

FBal0207607

Feature type

allele

Associated gene

Dmel\Mekk1

Associated Insertion(s)

Carried in Construct

P{GD9945}

Also Known As

UAS-MEKK1-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD9945}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD9945

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with Scer\GAL4^da.G32

(Ha et al., 2009)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

The recovery from the midgut epithelium thinning induced by infection with Serratia marcescens occurs normally in adult flies expressing Mekk1^GD9945 under the control of Scer\GAL4^NP3084 (together with tub-Gal80[ts] to limit the time of expression).

(Lee et al., 2016)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or early pupal lethality.

(Schnorrer et al., 2010)

Flies expressing Mekk1^GD9945 under the control of Scer\GAL4^da.G32 show reduced survival after gut infection (via feeding) with Erwinia carotovora carotovora strain 15 compared to control flies.

(Ha et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9945

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Mekk1^GD9945, Scer\GAL4^da.G32 has abnormal immune response phenotype, suppressible by Duox^UAS.cHa, Scer\GAL4^da.G32

(Ha et al., 2009)

Suppressor of

Statement

Reference

Mekk1^GD9945, Scer\GAL4^cv-c-C5 is a suppressor of abnormal size | adult stage phenotype of Dad1^GD1006, Scer\GAL4^cv-c-C5

(Zhang et al., 2016)

Mekk1^GD9945, Scer\GAL4^cv-c-C5 is a suppressor of visible | adult stage phenotype of Dad1^GD1006, Scer\GAL4^cv-c-C5

(Zhang et al., 2016)

Mekk1^GD9945, Scer\GAL4^en.PU is a suppressor of increased cell death | third instar larval stage phenotype of Dad1^GD1006, Scer\GAL4^en.PU

(Zhang et al., 2016)

Phenotype Manifest In

Suppressor of

Statement

Reference

Mekk1^GD9945, Scer\GAL4^cv-c-C5 is a suppressor of wing phenotype of Dad1^GD1006, Scer\GAL4^cv-c-C5

(Zhang et al., 2016)

Mekk1^GD9945, Scer\GAL4^en.PU is a suppressor of wing disc | third instar larval stage phenotype of Dad1^GD1006, Scer\GAL4^en.PU

(Zhang et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The small adult wing size along with the increased apoptosis in the third instar larval wing discs induced upon expression of Dad1^GD1006 driven by Scer\GAL4^C5 and Scer\GAL4^en.PU, respectively, is suppressed by co-expression of Mekk1^GD9945.

(Zhang et al., 2016)

The reduced survival of flies expressing Mekk1^GD9945 under the control of Scer\GAL4^da.G32 that have been fed Erwinia carotovora carotovora strain 15 is suppressed by co-expression of Duox^Scer\UAS.cHa.

(Ha et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v25529

w¹¹¹⁸; P{GD9945}v25529/TM3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9945

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Mekk1^GD9945

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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