FlyBase Allele Report: Dmel\Met[GD4384]

General Information

Symbol

Dmel\Met^GD4384

Species

D. melanogaster

Name

FlyBase ID

FBal0207612

Feature type

allele

Associated gene

Dmel\Met

Associated Insertion(s)

Carried in Construct

P{GD4384}

Also Known As

Met RNAi, UAS-Met-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4384}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4384

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Adults expressing Met^GD4384 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in a weak flyer phenotype.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4384

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL80^ts.αTub84B, Met^GD4384, Scer\GAL4^da.PU is a non-enhancer of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL80^ts.αTub84B, Met^GD4384, Scer\GAL4^da.PU is a non-suppressor of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Other

Statement

Reference

Met^GD4384, Scer\GAL4^Pde8-1246-G4, gce^KK109764 has male fertile phenotype

(Kurogi et al., 2024)

Met^GD4384, Scer\GAL4^Act5C.PI, gce^2.5k has majority die during P-stage phenotype

(Baumann et al., 2017)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

gce^2.5k mutants expressing Met^GD4384 under the control of Scer\GAL4^Act5C.PI exhibit nearly 100% pupal lethality.

(Baumann et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v45854

w¹¹¹⁸; P{GD4384}v45854

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4384

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Met^GD4384

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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