UASt regulatory sequences drive expression of an inverted repeat.
abnormal pain response | larval stage, with Scer\GAL41003.3, Scer\GAL80tsh-GAL80
abnormal pain response | larval stage, with Scer\GAL4ppk.PG
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Expression of sNPF-RGD661 under the control of Scer\GAL41003.3 and Scer\GAL80tsh-GAL80 or Scer\GAL4ppk.PG in larvae significantly reduces nociceptive movement responses following stimulation with a von Frey filament, compared to controls.
Adults expressing sNPF-RGD661 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.