FlyBase Allele Report: Scer\GAL4[1003.3]

General Information

Symbol

Scer\GAL4^1003.3

Species

S. cerevisiae

Name

FlyBase ID

FBal0240969

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GawB}1003.3

Carried in Construct

Also Known As

1003.3-GAL4, 1003.3

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Mobilisation of an X-linked P{GawB} generates this line.

(Hughes and Thomas, 2007)

Insertion components

P{GawB}1003.3

Product class / Tool use(s)

Encoded product / tool

enhancer trap

GAL4

binary expression system - driver

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

abdominal dorsal multidendritic neuron ddaC

(Shimono et al., 2009)

abdominal lateral multidendritic neuron ldaA

(Shimono et al., 2009)

abdominal anterior ventral multidendritic neuron vdaa

(Shimono et al., 2009)

adult abdominal lateral multidendritic neuron ldaA-like

(Shimono et al., 2009)

Additional Information

Statement

Reference

Scer\GAL4^1003.3 may also drive expression in abdominal dorsal multidendritic neurons ddaD and ddaE.

(Shimono et al., 2009)

Scer\GAL4^1003.3 drives expression in the PNS neurons, including class I and class II dendritic arborizing neurons, external sensory organs and the vch1 and vch2 chordotonal organs. Labelling is also seen in the lateral ventral nerve cord and a few cells in the brain.

(Hughes and Thomas, 2007)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

behavior defective | larval stage | chemical conditional, with Crei\ChR2^UAS.cHa.EYFP

(Tsubouchi et al., 2012)

behavior defective | third instar larval stage | chemical conditional, with Crei\ChR2^UAS.cHa.EYFP

(Hwang et al., 2007)

locomotor behavior defective | larval stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL80^tsh-GAL80

(Hu et al., 2017)

neuroanatomy defective | larval stage, with Cdc42^N17.UAS

(Tsubouchi et al., 2012)

neuroanatomy defective | larval stage, with Dcr-2^UAS.cDa, mri^GD7508

(Tsubouchi et al., 2012)

neuroanatomy defective | larval stage, with Dcr-2^UAS.cDa, na^GD1172

(Tsubouchi et al., 2012)

neuroanatomy defective | larval stage, with Dcr-2^UAS.cDa, Nmdar1^GD2808

(Tsubouchi et al., 2012)

neuroanatomy defective | larval stage, with Dcr-2^UAS.cDa, Nmdar2^GD3192

(Tsubouchi et al., 2012)

neuroanatomy defective | larval stage, with Dcr-2^UAS.cDa, nompC^KK105819

(Tsubouchi et al., 2012)

neuroanatomy defective | larval stage, with Dcr-2^UAS.cDa, rpk^GD3621

(Tsubouchi et al., 2012)

neuroanatomy defective | larval stage, with Rac1^N17.UAS

(Tsubouchi et al., 2012)

neuroanatomy defective | larval stage, with Rac1^UAS.cLa

(Tsubouchi et al., 2012)

pain response defective | larval stage, with Ctet\tetX^TNT-LC.UAS, Scer\GAL80^tsh-GAL80

(Hu et al., 2017)

pain response defective | larval stage, with Hsap\KCNJ2^UAS.EGFP, Scer\GAL80^tsh-GAL80

(Hu et al., 2017)

pain response defective | larval stage, with Scer\GAL80^tsh-GAL80, sNPF-R^DN.UAS

(Hu et al., 2017)

pain response defective | larval stage, with Scer\GAL80^tsh-GAL80, sNPF-R^GD661

(Hu et al., 2017)

pain response defective | larval stage, with Scer\GAL80^tsh-GAL80, sNPF-R^JF02657

(Hu et al., 2017)

touch response defective, with ClC-b^GD1958

(Tsubouchi et al., 2012)

touch response defective, with mri^GD7508

(Tsubouchi et al., 2012)

touch response defective, with na^GD1172

(Tsubouchi et al., 2012)

touch response defective, with Nmdar1^KK107519

(Tsubouchi et al., 2012)

touch response defective, with Nmdar2^GD3192

(Tsubouchi et al., 2012)

touch response defective, with nompC^KK105819

(Tsubouchi et al., 2012)

touch response defective, with rpk^GD3621

(Tsubouchi et al., 2012)

touch response defective | larval stage, with Cdc42^N17.UAS

(Tsubouchi et al., 2012)

touch response defective | larval stage, with ClC-b^GD1958, Dcr-2^UAS.cDa

(Tsubouchi et al., 2012)

touch response defective | larval stage, with Ctet\tetX^TNT-LC.UAS

(Tsubouchi et al., 2012)

touch response defective | larval stage, with Dcr-2^UAS.cDa, mri^GD7508

(Tsubouchi et al., 2012)

touch response defective | larval stage, with Dcr-2^UAS.cDa, na^GD1172

(Tsubouchi et al., 2012)

touch response defective | larval stage, with Dcr-2^UAS.cDa, Nmdar1^GD2808

(Tsubouchi et al., 2012)

touch response defective | larval stage, with Dcr-2^UAS.cDa, Nmdar2^GD3192

(Tsubouchi et al., 2012)

touch response defective | larval stage, with Dcr-2^UAS.cDa, nompC^KK105819

(Tsubouchi et al., 2012)

touch response defective | larval stage, with Dcr-2^UAS.cDa, rpk^GD3621

(Tsubouchi et al., 2012)

touch response defective | larval stage, with Hsap\KCNJ2^UAS.EGFP, Scer\GAL80^tsh-GAL80

(Hu et al., 2017)

touch response defective | larval stage, with Rac1^N17.UAS

(Tsubouchi et al., 2012)

touch response defective | larval stage, with Rac1^UAS.cLa

(Tsubouchi et al., 2012)

Phenotype Manifest In

larval dorsal multidendritic neuron ddaA | larval stage, with Cdc42^N17.UAS

(Tsubouchi et al., 2012)

larval dorsal multidendritic neuron ddaA | larval stage, with Dcr-2^UAS.cDa, mri^GD7508

(Tsubouchi et al., 2012)

larval dorsal multidendritic neuron ddaA | larval stage, with Dcr-2^UAS.cDa, na^GD1172

(Tsubouchi et al., 2012)

larval dorsal multidendritic neuron ddaA | larval stage, with Dcr-2^UAS.cDa, Nmdar1^GD2808

(Tsubouchi et al., 2012)

larval dorsal multidendritic neuron ddaA | larval stage, with Dcr-2^UAS.cDa, Nmdar2^GD3192

(Tsubouchi et al., 2012)

larval dorsal multidendritic neuron ddaA | larval stage, with Dcr-2^UAS.cDa, nompC^KK105819

(Tsubouchi et al., 2012)

larval dorsal multidendritic neuron ddaA | larval stage, with Dcr-2^UAS.cDa, rpk^GD3621

(Tsubouchi et al., 2012)

larval dorsal multidendritic neuron ddaA | larval stage, with Rac1^N17.UAS

(Tsubouchi et al., 2012)

larval dorsal multidendritic neuron ddaA | larval stage, with Rac1^UAS.cLa

(Tsubouchi et al., 2012)

sensory dendrite | larval stage, with Cdc42^N17.UAS

(Tsubouchi et al., 2012)

sensory dendrite | larval stage, with Dcr-2^UAS.cDa, mri^GD7508

(Tsubouchi et al., 2012)

sensory dendrite | larval stage, with Dcr-2^UAS.cDa, na^GD1172

(Tsubouchi et al., 2012)

sensory dendrite | larval stage, with Dcr-2^UAS.cDa, Nmdar1^GD2808

(Tsubouchi et al., 2012)

sensory dendrite | larval stage, with Dcr-2^UAS.cDa, Nmdar2^GD3192

(Tsubouchi et al., 2012)

sensory dendrite | larval stage, with Dcr-2^UAS.cDa, nompC^KK105819

(Tsubouchi et al., 2012)

sensory dendrite | larval stage, with Dcr-2^UAS.cDa, rpk^GD3621

(Tsubouchi et al., 2012)

sensory dendrite | larval stage, with Rac1^N17.UAS

(Tsubouchi et al., 2012)

sensory dendrite | larval stage, with Rac1^UAS.cLa

(Tsubouchi et al., 2012)

Detailed Description

Statement

Reference

Inhibition (using Hsap\KCNJ2^UAS.EGFP) of a subset of Scer\GAL4^1003.3 cells (inhibition restricted using Scer\GAL80^tsh-GAL80) in larvae significantly reduces nociceptive movement responses following stimulation with a von Frey filament, compared to controls; there is also a significant reduction in the response to gentle touch, compared to controls.

Inhibition (using Ctet\tetX^TNT-LC.UAS) of a subset of Scer\GAL4^1003.3 cells (activation restricted using Scer\GAL80^tsh-GAL80) in larvae significantly reduces nociceptive movement responses following stimulation with a von Frey filament, but does not affect the latency of rolling in response to high temperature (local) stimulation, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 625 nm (red) light) of a subset of Scer\GAL4^1003.3 cells (activation restricted using Scer\GAL80^tsh-GAL80) in larvae results in contractions without rolling in the absence of pain stimuli, this is not seen in controls.

(Hu et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

Smn^UAS.Tag:FLAG/Scer\GAL4^1003.3 fails to rescue Smn^X7

(Imlach et al., 2012)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^1003.3

Name Synonyms

Secondary FlyBase IDs

References (8)

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