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General Information
Symbol
Dmel\SmnUAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0241133
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Flag-Smn, UAS-SMN
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of Smn which is tagged at the N-terminal end with Tag:FLAG.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of SmnScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4Mef2.PR has no effect on flight ability of adult flies, mobility of the third instar larvae or shape of pupae and Scer\GAL4elav-C155-driven expression does not impair adult viability.

Expression of SmnScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PU leads to mild defects in eye morphology.

Expression of SmnScer\UAS.T:Zzzz\FLAG driven by Scer\GAL4Mef2.PR does not significantly affect flight behavior.

Overexpression of SmnScer\UAS.T:Zzzz\FLAG driven by Scer\GAL4elav-C155, Scer\GAL4how-24B, Scer\GAL4VGlut-OK371 or Scer\GAL4ChAT.7.4 does not significantly disrupt locomotor behavior (speed, distance covered, overall activity, pause number and length in Buridan's assay) in adult flies compared to wild type.

Baseline synaptic transmission measured at the neuromuscular junction (muscle 6/7 in abdominal segment 2) is mildly but significantly altered in third instar larvae with SmnScer\UAS.T:Zzzz\FLAG driven by Scer\GAL4elav-C155 : though no changes in excitatory junction potential (EJP) amplitude or the quantal content of release were detected, there is a significant increase in mini evoked junction potential (mEJP) amplitude. When SmnScer\UAS.T:Zzzz\FLAG is driven in muscles by Scer\GAL4how-24B there are significant decreases in EJP and mEJP amplitude.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Enhancer of
Additional Comments
Genetic Interactions
Statement
Reference

Simultaneous expression of Gem3ΔN.Scer\UAS and SmnScer\UAS.T:Zzzz\FLAG leads to strongly reduced mobility in third instar larvae and more elongated shape of the puparium with the Scer\GAL4Mef2.PR driver and reduced adult viability with Scer\GAL4elav-C155.

Co-expression of SmnScer\UAS.T:Zzzz\FLAG and Gem3ΔN.Scer\UAS driven by Scer\GAL4Mef2.PR results in partial lethality, with the few escapers exhibiting severe flight defects.

Xenogenetic Interactions
Statement
Reference

Co-expression of SmnScer\UAS.T:Zzzz\FLAG does not modify the mild eye defects seen in flies expressing Hsap\FUSMM.Scer\UAS.T:Zzzz\FLAG or Hsap\FUSScer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PU.

Complementation and Rescue Data
Comments

Expression of SmnScer\UAS.T:Zzzz\FLAG under the control of either Scer\GAL4Act.PU or Scer\GAL4tub.PU partially rescues the semi-lethality seen when one copy each of SmnT:Zzzz\FLAG and SmnY203C.T:Zzzz\FLAG are expressed in a homozygous SmnX7 mutant background.

Decreased muscle surface area, locomotor defects, increased inter-spike intervals and increased evoked excitatory postsynaptic potential (eEPSP) amplitude in SmnX7/SmnX7 third instar larvae are rescued by ubiquitous (Scer\GAL4da.PU), pan-neuronal (Scer\GAL4nSyb.PS) or cholinergic (Scer\GAL4ChAT.7.4) expression of SmnScer\UAS.T:Zzzz\FLAG; these phenotypes are not rescued when expression is driven by larval muscle-specific Scer\GAL4G14, glutamatergic Scer\GAL4VGlut-OK371, motor-neuron specific Scer\GAL4RapGAP1-OK6, GABAergic (Scer\GAL4Gad1.3.098), Scer\GAL41003.3 or Scer\GAL4ppk.1.9. Expression of SmnScer\UAS.T:Zzzz\FLAG with Scer\GAL4clh201 or Scer\GAL4NP2225 rescues the increased inter-spike intervals and increased eEPSP amplitude, partially rescues the decreased muscle surface area, but fails to rescue the locomotion defect in SmnX7/SmnX7 third instar larvae.

SmnScer\UAS.T:Zzzz\FLAG driven by Scer\GAL4elav.Switch.PO in the presence of RU486 rescues SmnX7/SmnX7 muscle size, locomotion (partially), inter-spike intervals and eEPSP defects if SmnScer\UAS.T:Zzzz\FLAG expression is induced immediately after hatching (if induced 48 or 96 hours after hatching rescue is partial apart from full rescue of eEPSP).

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
SmnScer\UAS.T:Zzzz\FLAG
SmnUAS.Tag:FLAG
Name Synonyms
Secondary FlyBase IDs
    References (10)