Simultaneous expression of Gem3ΔN.Scer\UAS and SmnScer\UAS.T:Zzzz\FLAG leads to strongly reduced mobility in third instar larvae and more elongated shape of the puparium with the Scer\GAL4Mef2.PR driver and reduced adult viability with Scer\GAL4elav-C155.
Expression of SmnScer\UAS.T:Zzzz\FLAG under the control of either Scer\GAL4Act.PU or Scer\GAL4tub.PU partially rescues the semi-lethality seen when one copy each of SmnT:Zzzz\FLAG and SmnY203C.T:Zzzz\FLAG are expressed in a homozygous SmnX7 mutant background.
Decreased muscle surface area, locomotor defects, increased inter-spike intervals and increased evoked excitatory postsynaptic potential (eEPSP) amplitude in SmnX7/SmnX7 third instar larvae are rescued by ubiquitous (Scer\GAL4da.PU), pan-neuronal (Scer\GAL4nSyb.PS) or cholinergic (Scer\GAL4ChAT.7.4) expression of SmnScer\UAS.T:Zzzz\FLAG; these phenotypes are not rescued when expression is driven by larval muscle-specific Scer\GAL4G14, glutamatergic Scer\GAL4VGlut-OK371, motor-neuron specific Scer\GAL4RapGAP1-OK6, GABAergic (Scer\GAL4Gad1.3.098), Scer\GAL41003.3 or Scer\GAL4ppk.1.9. Expression of SmnScer\UAS.T:Zzzz\FLAG with Scer\GAL4clh201 or Scer\GAL4NP2225 rescues the increased inter-spike intervals and increased eEPSP amplitude, partially rescues the decreased muscle surface area, but fails to rescue the locomotion defect in SmnX7/SmnX7 third instar larvae.
SmnScer\UAS.T:Zzzz\FLAG driven by Scer\GAL4elav.Switch.PO in the presence of RU486 rescues SmnX7/SmnX7 muscle size, locomotion (partially), inter-spike intervals and eEPSP defects if SmnScer\UAS.T:Zzzz\FLAG expression is induced immediately after hatching (if induced 48 or 96 hours after hatching rescue is partial apart from full rescue of eEPSP).