FB2021_02, released April 13, 2021

Allele: Hsap\FUSMM.UAS.Tag:FLAG

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General Information
Symbol
Hsap\FUSMM.UAS.Tag:FLAG
Species
H. sapiens
Name
FlyBase ID
FBal0324784
Feature type
allele
Associated gene
Hsap\FUS
Associated Insertion(s)
Carried in Construct
M{UAS-Flag-HsapFUS.MM}
Also Known As
Flag-FUSMM
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Di Salvio et al., 2015)
in vitro construct - coding region fusion
(Di Salvio et al., 2015)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      M{UAS-Flag-HsapFUS.MM}
      Cytology
      Nature of the lesion
      Statement
      Reference

      UASt regulatory sequences drive expression of Hsap\FUS carrying four mutations (R521G, R522G, R524S, and P525L) that have been associated with amyotrophic lateral sclerosis. The protein is tagged with Tag:FLAG.

      (Di Salvio et al., 2015)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UASt
      Encoded product / tool
      Hsap\FUS
      Tagged with
      Tag:FLAG
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  amyotrophic lateral sclerosis type 6
      CEA
      (Di Salvio et al., 2015)
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      model of  amyotrophic lateral sclerosis type 6
      is ameliorated by Pur-αKK108663
      (Di Salvio et al., 2015)
      is exacerbated by Rnor\PuraUAS.Tag:HA
      (Di Salvio et al., 2015)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Hsap\FUSMM.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PU leads to mild defects in eye morphology.

      (Mirra et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      NOT Enhanced by
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of SmnScer\UAS.T:Zzzz\FLAG or SmnHMC03832 does not modify the mild eye defects seen in flies expressing Hsap\FUSMM.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4GMR.PU.

      (Mirra et al., 2017)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Hsap\FUSMM.Scer\UAS.T:Zzzz\FLAG
      Hsap\FUSMM.UAS.Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
        References (2)