FlyBase Allele Report: Dmel\Smn[Tag:FLAG]

General Information

Symbol

Dmel\Smn^Tag:FLAG

Species

D. melanogaster

Name

FlyBase ID

FBal0277063

Feature type

allele

Associated gene

Dmel\Smn

Associated Insertion(s)

Carried in Construct

M{Smn-FLAG}

Also Known As

Smn^WT, Flag-Smn^WT

Key Links

Transgenic product class

wild_type

(Praveen et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Praveen et al., 2012)

Mutagen

in vitro construct

(Praveen et al., 2012)

Progenitor genotype

Carried in construct

M{Smn-FLAG}

Cytology

Description

A ~3kb fragment containing the entire Smn coding region is tagged with Tag:FLAG.

(Praveen et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

Smn

Encoded product / tool

Smn

Tagged with

Tag:FLAG

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Smn^Tag:FLAG rescues Smn^X7

(Gupta et al., 2021)

Smn^{Y203C.Tag:FLAG}, Smn^Tag:FLAG, Scer\GAL4^Act.PU, Smn^UAS.Tag:FLAG rescues Smn^X7

(Praveen et al., 2014)

Partially rescues

Smn^{F70S.Tag:FLAG}/Smn^Tag:FLAG partially rescues Smn^X7

(Gupta et al., 2021)

Smn^Tag:FLAG/Smn^{Y208C.Tag:FLAG} partially rescues Smn^X7

(Gupta et al., 2021)

Smn^Tag:FLAG/Smn^{G210V.Tag:FLAG} partially rescues Smn^X7

(Gupta et al., 2021)

Smn^Tag:FLAG/Smn^{V72G.Tag:FLAG} partially rescues Smn^X7

(Gupta et al., 2021)

Smn^Tag:FLAG partially rescues Smn^X7

(Gray et al., 2018)

Smn^Tag:FLAG/Smn^{M194R.Tag:FLAG} partially rescues Smn^X7

(Praveen et al., 2014)

Smn^{Y203C.Tag:FLAG}/Smn^Tag:FLAG partially rescues Smn^X7

(Praveen et al., 2014)

Smn^Tag:FLAG/Smn^{G206S.Tag:FLAG} partially rescues Smn^X7

(Praveen et al., 2014)

Smn^Tag:FLAG partially rescues Smn^X7

(Praveen et al., 2014)

Smn^Tag:FLAG/Smn^{G210V.Tag:FLAG} partially rescues Smn^X7

(Praveen et al., 2014)

Smn^Tag:FLAG/Smn^{V72G.Tag:FLAG} partially rescues Smn^X7

(Praveen et al., 2014)

Smn^Tag:FLAG partially rescues Smn^X7/Smn^f01109

(Praveen et al., 2012)

Comments

Smn^Tag:FLAG rescues both the larval locomotor defects and the larval lethality of Smn^X7 homozygotes, although individuals show a small decrease in viability compared to controls.

(Gray et al., 2018)

Expression of Smn^T:Zzzz\FLAG significantly rescues the lethal lethality seen in homozygous Smn^X7 mutants, resulting in 67% adult viability.

Co-expression of one copy each of Smn^T:Zzzz\FLAG and Smn^{M194R.T:Zzzz\FLAG} partially rescues the larval lethality seen in homozygous Smn^X7 mutants. The phenotype is intermediate between expressing one copy each of either transgene, indicating that Smn^{M194R.T:Zzzz\FLAG} is having a dominant effect.

Co-expression of one copy each of Smn^T:Zzzz\FLAG and Smn^{Y203C.T:Zzzz\FLAG} partially rescues the larval lethality seen in homozygous Smn^X7 mutants. The phenotype is intermediate between expressing one copy each of either transgene, indicating that Smn^{Y203C.T:Zzzz\FLAG} is having a dominant effect.

Co-expression of one copy each of Smn^T:Zzzz\FLAG and Smn^{G206S.T:Zzzz\FLAG} partially rescues the larval lethality seen in homozygous Smn^X7 mutants. The phenotype is intermediate between expressing one copy each of either transgene, indicating that Smn^{G206S.T:Zzzz\FLAG} is having a dominant effect.

Co-expression of one copy each of Smn^T:Zzzz\FLAG and Smn^{G210V.T:Zzzz\FLAG} partially rescues the larval lethality seen in homozygous Smn^X7 mutants. The phenotype is intermediate between expressing one copy each of either transgene, indicating that Smn^{G210V.T:Zzzz\FLAG} is having a dominant effect. A few adults eclose but most animals reach the pupal stage.

Co-expression of one copy each of Smn^T:Zzzz\FLAG and Smn^{V72G.T:Zzzz\FLAG} partially rescues the larval lethality seen in homozygous Smn^X7 mutants. The phenotype is intermediate between expressing one copy each of either transgene, with many adults eclosing but fewer than when the wild type transgene is expressed alone. This indicates that Smn^{V72G.T:Zzzz\FLAG} is having a mild dominant effect.

Expression of Smn^{Scer\UAS.T:Zzzz\FLAG} under the control of either Scer\GAL4^Act.PU or Scer\GAL4^tub.PU partially rescues the semi-lethality seen when one copy each of Smn^T:Zzzz\FLAG and Smn^{Y203C.T:Zzzz\FLAG} are expressed in a homozygous Smn^X7 mutant background.

(Praveen et al., 2014)

Expression of Smn^T:Zzzz\FLAG in a Smn^f01109/Smn^X7 mutant background results in >70% rescue of lethality. The resulting adults are fertile with no apparent defects in flight or motility. Pupation and eclosion occurs a day later than in wild type controls. Expression of Smn^T:Zzzz\FLAG also rescues the motility and burrowing defects seen in Smn^f01109/Smn^X7 mutant larvae.

(Praveen et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Smn^T:Zzzz\FLAG

Smn^Tag:FLAG

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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