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General Information
Symbol
Dmel\SmnTag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0277063
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
SmnWT, Flag-SmnWT
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A ~3kb fragment containing the entire Smn coding region is tagged with Tag:FLAG.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of SmnT:Zzzz\FLAG significantly rescues the lethal lethality seen in homozygous SmnX7 mutants, resulting in 67% adult viability.

Co-expression of one copy each of SmnT:Zzzz\FLAG and SmnM194R.T:Zzzz\FLAG partially rescues the larval lethality seen in homozygous SmnX7 mutants. The phenotype is intermediate between expressing one copy each of either transgene, indicating that SmnM194R.T:Zzzz\FLAG is having a dominant effect.

Co-expression of one copy each of SmnT:Zzzz\FLAG and SmnY203C.T:Zzzz\FLAG partially rescues the larval lethality seen in homozygous SmnX7 mutants. The phenotype is intermediate between expressing one copy each of either transgene, indicating that SmnY203C.T:Zzzz\FLAG is having a dominant effect.

Co-expression of one copy each of SmnT:Zzzz\FLAG and SmnG206S.T:Zzzz\FLAG partially rescues the larval lethality seen in homozygous SmnX7 mutants. The phenotype is intermediate between expressing one copy each of either transgene, indicating that SmnG206S.T:Zzzz\FLAG is having a dominant effect.

Co-expression of one copy each of SmnT:Zzzz\FLAG and SmnG210V.T:Zzzz\FLAG partially rescues the larval lethality seen in homozygous SmnX7 mutants. The phenotype is intermediate between expressing one copy each of either transgene, indicating that SmnG210V.T:Zzzz\FLAG is having a dominant effect. A few adults eclose but most animals reach the pupal stage.

Co-expression of one copy each of SmnT:Zzzz\FLAG and SmnV72G.T:Zzzz\FLAG partially rescues the larval lethality seen in homozygous SmnX7 mutants. The phenotype is intermediate between expressing one copy each of either transgene, with many adults eclosing but fewer than when the wild type transgene is expressed alone. This indicates that SmnV72G.T:Zzzz\FLAG is having a mild dominant effect.

Expression of SmnScer\UAS.T:Zzzz\FLAG under the control of either Scer\GAL4Act.PU or Scer\GAL4tub.PU partially rescues the semi-lethality seen when one copy each of SmnT:Zzzz\FLAG and SmnY203C.T:Zzzz\FLAG are expressed in a homozygous SmnX7 mutant background.

Expression of SmnT:Zzzz\FLAG in a Smnf01109/SmnX7 mutant background results in >70% rescue of lethality. The resulting adults are fertile with no apparent defects in flight or motility. Pupation and eclosion occurs a day later than in wild type controls. Expression of SmnT:Zzzz\FLAG also rescues the motility and burrowing defects seen in Smnf01109/SmnX7 mutant larvae.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
SmnT:Zzzz\FLAG
SmnTag:FLAG
Name Synonyms
Secondary FlyBase IDs
    References (5)