FlyBase Allele Report: Dmel\Rx[GD4076]

General Information

Symbol

Dmel\Rx^GD4076

Species

D. melanogaster

Name

FlyBase ID

FBal0208431

Feature type

allele

Associated gene

Dmel\Rx

Associated Insertion(s)

Carried in Construct

P{GD4076}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4076}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4076

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

abnormal neuroanatomy | P-stage, with Scer\GAL4^insc-Mz1407

(Kraft et al., 2016)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Kraft et al., 2016)

decreased cell number | P-stage, with Scer\GAL4^insc-Mz1407

(Kraft et al., 2016)

decreased cell number | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Kraft et al., 2016)

decreased cell size | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Kraft et al., 2016)

decreased occurrence of cell division | third instar larval stage, with Scer\GAL4^insc-Mz1407

(Kraft et al., 2016)

lethal, with Scer\GAL4^insc-Mz1407

(Neumüller et al., 2011)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Rx^GD4076 under the control of Scer\GAL4^insc-Mz1407 results in a significant decrease in the number of mushroom body neuroblasts and Kenyon cells in the mushroom body at 72 h after puparium formation, a decrease in the number of mushroom body GMCs at the third instar stage, a reduction in proliferation of mushroom body neuroblasts and GMCs in late third instar brains, and a decrease in third instar mushroom body neuroblast and GMC size, as compared to controls.

(Kraft et al., 2016)

Expression under the control of Scer\GAL4¹⁴⁰⁷ results in shorter neuroblast lineages (less daughter cells per neuroblast) in the larval brain compared to controls.

(Neumüller et al., 2011)

Adults expressing Rx^GD4076 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 10-20% or 30-40% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 30-40% or 90-100% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4076

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Rx^GD4076, Scer\GAL4^insc-Mz1407 has decreased cell number | P-stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^insc-Mz1407

(Kraft et al., 2016)

Rx^GD4076, Scer\GAL4^insc-Mz1407 has abnormal neuroanatomy | P-stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^insc-Mz1407

(Kraft et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Rx^GD4076, Scer\GAL4^insc-Mz1407 has neuroblast MBp | P-stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^insc-Mz1407

(Kraft et al., 2016)

Rx^GD4076, Scer\GAL4^insc-Mz1407 has mushroom body | P-stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^insc-Mz1407

(Kraft et al., 2016)

Rx^GD4076, Scer\GAL4^insc-Mz1407 has Kenyon cell | P-stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^insc-Mz1407

(Kraft et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa restores the number of mushroom body neuroblasts, and partially restores the number of Kenyon cells to normal in flies expressing Rx^GD4076 under the control of Scer\GAL4^insc-Mz1407 at 72 h APF.

(Kraft et al., 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v44716

w¹¹¹⁸; P{GD4076}v44716

Notes on Origin

Discoverer