FlyBase Allele Report: Dmel\VGlut1[GD834]

General Information

Symbol

Dmel\VGlut1^GD834

Species

D. melanogaster

Name

FlyBase ID

FBal0208956

Feature type

allele

Associated gene

Dmel\VGlut1

Associated Insertion(s)

Carried in Construct

P{GD834}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD834}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD834

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurodegenerative disease

modeled by mir-1000^KO2, mir-1000^KO2-GAL4

(Verma et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Third instar larvae expressing VGlut^GD834 under the control of Scer\GAL4^nSyb.PU do not show changes in head bending behavior during crawling, as compared to controls.

(Otto et al., 2018)

Expression of VGlut^GD834 (co-expressed with Dcr-2^Scer\UAS.cDa) in either dm8 neurons (under the control of Scer\GAL4^ort.C2b) or Tm5c neurons (under the control of Scer\GAL4^{C1a.DBD.ort.T:Zzzz\ZipRREEL} and Hsim\VP16^{AD.VGlut.OK371.T:Zzzz\ZipEERRL,T:SV40\nls2}) significantly reduces the phototactic preference for ultraviolet light over green light seen in wild type and control flies.

(Karuppudurai et al., 2014)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^repo/VGlut1^GD834 is a suppressor | partially of abnormal neurophysiology | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Scer\GAL4^VGlut1-OK371/VGlut1^GD834 is a suppressor | partially of abnormal neurophysiology | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Scer\GAL4^VGlut1-OK371/VGlut1^GD834 is a suppressor | partially of abnormal locomotor behavior | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

VGlut1^GD834/Scer\GAL4^{mir-1000-KO2-GAL4} is a suppressor | partially of short lived phenotype of mir-1000^KO2-GAL4/mir-1000^KO2

(Verma et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^repo/VGlut1^GD834 is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Scer\GAL4^VGlut1-OK371/VGlut1^GD834 is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of Eaat1^hypo

(Peng et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

The climbing defects of two day old mir-1000^KO2/mir-1000^KO2-GAL4 adults are rescued by expression of VGlut^GD834 under the control of Scer\GAL4^{mir-1000-KO2-GAL4}. The amount of Caspase-3 positive cells in the brain is reduced, and lifespan is increased (although not to control levels).

(Verma et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v2574

w¹¹¹⁸; P{GD834}v2574/TM3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

VGlut1^GD834

VGlut^GD834

dvglut^RNAi

(Scholz et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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