FlyBase Allele Report: Dmel\bru1[GD17093]

General Information

Symbol

Dmel\bru1^GD17093

Species

D. melanogaster

Name

FlyBase ID

FBal0209072

Feature type

allele

Associated gene

Dmel\bru1

Associated Insertion(s)

Carried in Construct

P{GD17093}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD17093}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD17093

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal flight, with Scer\GAL4^Act88F.PB

(Oas et al., 2014)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

flight muscle cell, with Scer\GAL4^Act88F.PB

(Oas et al., 2014)

myofibril, with Scer\GAL4^Act88F.PB

(Oas et al., 2014)

sarcomere, with Scer\GAL4^Act88F.PB

(Oas et al., 2014)

Z disc, with Scer\GAL4^Act88F.PB

(Oas et al., 2014)

Detailed Description

Statement

Reference

Flies expressing aret^GD17093 under the control of Scer\GAL4^Act88F.PB have significant defects in flight ability compared to wild type.

The size and position of the flight muscles are normal in adults expressing aret^GD17093 under the control of Scer\GAL4^Act88F.PB. However, the myofibrils of these muscles are irregular in shape. Ultrastructurally, normal sarcomere structure is lost, with fuzzy and penetrated Z lines, diffuse myofibril boundaries and loose packing of myofilaments (allowing incorporation of mitochondria and other membraneous structures into the vicinity of the myofibril). Thin and thick filaments can be identified, but the normal hexagonal lattice organisation is lost.

(Oas et al., 2014)

Expression under the control of Scer\GAL4^Mef2.PR results in undefined lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 30-40% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

17093

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v48237

w¹¹¹⁸; P{GD17093}v48237/TM3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

17093

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

aret^GD17093

bru1^GD17093

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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