Down-regulation of dup in the subperineurial glia, through expression of dup^GD13203 under the control of Scer\GAL4^moody.PS inhibits DNA replication and results in a reduction in the number of subperineurial glia that are polypolid. This is concomitant with a cell size decrease.

Expression of dup^GD13203 in the subperineurial glia under the control of either Scer\GAL4^moody.PS (on chromosome II or III), or Scer\GAL4^Mz97 reduces polyploidization in subperineurial glia and increases dye penetration around the neuropil and the neuroblasts, indicating increase porosity of the blood-brain barrier in these flies. Amino acid starvation rescues both the dye penetration defect and septate junction pattern defects.

Expression of dup^GD13203 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in partial lethality or complete lethality, depending on the particular P{GD13203} insertion line used.

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in late larval lethality or late pupal lethality.

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage.

Scer\GAL4^moody.PS, dup^GD13203 has abnormal endomitotic cell cycle phenotype, suppressible by Myc^UAS.cZa, Scer\GAL4^moody.PS

Scer\GAL4^moody.PS, dup^GD13203 has decreased cell size phenotype, suppressible by Myc^UAS.cZa, Scer\GAL4^moody.PS