Expression of how^GD1462 specifically in early germ cells via Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in germline depletion and GSC loss in 37% of testes, whereas 27% lose some germline stem cells. These testes display an increase in the number of differentiated cyst cell nuclei near the hub.

Expression of how^GD1462 in early germ cells via Scer\GAL4^{nos.UTR.T:Hsim\VP16} results in some spermatogonial cysts not arresting at the 2-cell stage but differentiating early into 8-cell spermatocyte cysts. These cysts can complete meiosis and differentiate into cysts containing half of the normal complement of spermatids.

Expression of how^GD1462 in somatic stem cells and cyst cells does not result in any stem cell niche defects; however, the germ cells associated with maturing cyst cells are distinctly abnormal.

Adults expressing how^GD1462 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression under the control of Scer\GAL4^Mef2.PR results in early larval lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in sarcomeres with discontinuous Z-lines in the larval body wall muscles.

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage.