Expression of Inx6^GD16939 under the control of Scer\GAL4^ey-OK107 in adults does not significantly affect 3 hour aversive olfactory memory or the residual anaesthesia-resistant memory (ARM) after cold-shock, compared to controls; expression under the control of Scer\GAL4^VT000765 also does not affect ARM, compared to controls.

Expression of inx6^GD16939 in both the anterior paired lateral (APL) and dorsal paired medial (DPM) neurons in the mushroom body (using the Scer\GAL4^GH146 and Scer\GAL4^c316 drivers simultaneously, and in the presence of Dcr-2^Scer\UAS.cDa to increase RNAi efficiency) results in impaired 3 hour (intermediate-term) memory after associative conditioning compared to controls, although initial learning is normal in these flies. The anesthesia-resistant memory (ARM) component of intermediate-term memory is normal in these flies.

Impairment of 3 hour memory after associative conditioning is still seen in flies in which expression of inx6^GD16939 using the Scer\GAL4^GH146 and Scer\GAL4^c316 drivers simultaneously is limited to the adult stage by using the temperature sensitive Scer\GAL80^ts.αTub84B allele to restrict expression before this stage.

The gross anatomy of the APL and DPM neurons in flies expressing inx6^GD16939 using the Scer\GAL4^GH146 and Scer\GAL4^c316 drivers simultaneously is not different from that in control flies.

Expression of inx6^GD16939 in the DPM neurons under the control of Scer\GAL4⁵⁰¹⁵ results in impaired 3 hour memory after associative conditioning compared to controls.

Flies expressing inx6^GD16939 under the control of Scer\GAL4^GH146 have normal 3 hour memory after associative conditioning.