FlyBase Allele Report: Scer\GAL4[c316]

General Information

Symbol

Scer\GAL4^c316

Species

S. cerevisiae

Name

FlyBase ID

FBal0120631

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GawB}c316

Carried in Construct

Also Known As

c316-GAL4, c316, c316{GAL4}, Amn(c316)-Gal4, DPM-Gal4

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{GawB}c316

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{GawB}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

mushroom body dorsal paired medial cell

Comment: strong expression

(Wu et al., 2023, Higham et al., 2019, Chen et al., 2012, Lee et al., 2011, Pitman et al., 2011, Wu et al., 2011, Krashes et al., 2007, Waddell et al., 2000)

Kenyon cell | subset

(Wu et al., 2011)

adult brain | restricted

(Lee et al., 2011, Wu et al., 2011)

adult mushroom body alpha-lobe | restricted

(Pitman et al., 2011)

adult mushroom body beta-lobe | restricted

(Pitman et al., 2011)

mushroom body pedunculus | restricted

(Pitman et al., 2011)

neuron | subset of adult brain

(Chen et al., 2012)

adult mushroom body

Comment: medium expression

(Chen et al., 2012)

glial cell of adult brain

Comment: strong expression

(Chen et al., 2012)

eye

Comment: medium expression

(Chen et al., 2012)

adult mushroom body beta'-lobe | restricted

(Pitman et al., 2011)

adult mushroom body alpha'-lobe | restricted

(Pitman et al., 2011)

Additional Information

Statement

Reference

Drives expression in the mushroom body dorsal paired medial cell.

(FlyBase Curators, 2017)

ScerGAL4^c316 is expressed in several cells of the adult brain, including a subset of Kenyon cells and in the mushroom body dorsal paired medial neurons.

(Wu et al., 2011)

ScerGAL4^c316 is expressed in the mushroom body dorsal paired medial cell, and in that cell's dense arborization in the mushroom body alpha'/beta'-lobes. Use of GFP-reconstitution across synaptic partners (GRASP) reveals that there are close contacts between the mushroom body dorsal paired medial cell and the mushroom body neurons in the mushroom body lobes and pedunculus. The terminals of the mushroom body dorsal and anterior paired medial cells are in close association in the mushroom body alpha'/beta' lobes and in the pedunculus. Co-expression of ScerGAL4^c316 with a presynaptic marker preferentially labels the presynaptic terminals of the mushroom body dorsal paired medial cell in the mushroom body alpha/beta lobes.

(Pitman et al., 2011)

ScerGAL4^c316 is expressed in a subset of cells in the adult brain, including the mushroom body dorsal paired medial cells.

(Lee et al., 2011)

Marker for

mushroom body dorsal paired medial cell

(Wu et al., 2023, Lee et al., 2011, Pitman et al., 2011, Krashes et al., 2007)

Reflects expression of

amn

(Higham et al., 2019)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory, with amn^UAS.cWa, amn^X8

(Keene et al., 2004)

abnormal memory, with Ddc^GD1189, Scer\GAL80^ts.αTub84B

(Lee et al., 2011)

abnormal memory, with Inx6^GD3692, Scer\GAL4^GH146

(Wu et al., 2011)

abnormal memory, with Inx6^GD16939, Scer\GAL4^GH146

(Wu et al., 2011)

abnormal memory, with Inx7^KK112684, Scer\GAL4^GH146

(Wu et al., 2011)

abnormal memory, with shi^1.UAS

(Tonoki and Davis, 2015, Lee et al., 2011)

abnormal memory | adult stage, with KCNQ^JF02562

(Cavaliere et al., 2013)

abnormal memory | adult stage, with mir-980^{sponge.V2.UAS.mCherry}

(Guven-Ozkan et al., 2016)

abnormal memory | adult stage, with shi^1.UAS

(Krashes et al., 2007)

abnormal memory | heat sensitive, with Inx6^GD16939, Scer\GAL4^GH146, Scer\GAL80^ts.αTub84B

(Wu et al., 2011)

abnormal memory | heat sensitive, with Inx7^KK112684, Scer\GAL4^GH146, Scer\GAL80^ts.αTub84B

(Wu et al., 2011)

abnormal memory | heat sensitive, with shi^1.UAS

(Pitman et al., 2011, Keene et al., 2006, Yu et al., 2005, Waddell et al., 2000)

abnormal memory | temperature conditional, with shi^1.UAS

(Keene et al., 2004)

abnormal neuroanatomy, with Dscam1^{exon17.2.UAS.GFP}

(Keene et al., 2006)

abnormal sleep, with VGAT^GD1990

(Haynes et al., 2015)

Phenotype Manifest In

mushroom body dorsal paired medial cell, with Dscam1^{exon17.2.UAS.GFP}

(Keene et al., 2006)

mushroom body dorsal paired medial cell, with shi^1.UAS

(Keene et al., 2006)

Detailed Description

Statement

Reference

Inhibition (using Hsap\KCNJ2^UAS.EGFP) of Scer\GAL4^c316 cells, in combination with a Gal80[ts] transgene to restrict expression to the adult stage, does not affect food intake or climbing rate, compared to controls.

Thermogenetic activation (using TrpA1^UAS.(B).cKa at 30[o]C) of Scer\GAL4^c316 cells in adults does not affect food intake or climbing rate, compared to controls.

(Al-Anzi and Zinn, 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^c316, shi^1.UAS has abnormal memory | adult stage phenotype, non-enhanceable by Dscam1^{exon17.2.UAS.GFP}

(Krashes et al., 2007)

Dscam1^{exon17.2.UAS.GFP}, Scer\GAL4^c316 has abnormal memory | adult stage phenotype, non-enhanceable by Dscam1^{exon17.2.UAS.GFP}