FlyBase Allele Report: Dmel\mir-980[sponge.V2.UAS.mCherry]

General Information

Symbol

Dmel\mir-980^{sponge.V2.UAS.mCherry}

Species

D. melanogaster

Name

FlyBase ID

FBal0305033

Feature type

allele

Associated gene

Dmel\mir-980

Associated Insertion(s)

Carried in Construct

P{UAS-mCherry.mir-980.sponge.V2}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Fulga et al., 2015)

Progenitor genotype

Carried in construct

P{UAS-mCherry.mir-980.sponge.V2}

Cytology

Description

Twenty miRNA binding sites with mismatches at positions 9-12 (creating a "Sponge", designed to sequester miRNAs and thus knocking-down endogenous miRNA activity) are placed in the 3'UTR of mCherry under the control of 10 Gal4 UAS binding sites.

(Fulga et al., 2015)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

mir-980

Tagged with

mCherry

red fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates intellectual disability

modeled by Rbfox1^KK109100

(Guven-Ozkan et al., 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | adult stage, with Scer\GAL4^c316

(Guven-Ozkan et al., 2016)

abnormal memory | adult stage, with Scer\GAL4^elav-C155

(Guven-Ozkan et al., 2016)

abnormal memory | adult stage, with Scer\GAL4^ey-OK107

(Guven-Ozkan et al., 2016)

abnormal memory | adult stage, with Scer\GAL4^GH146

(Guven-Ozkan et al., 2016)

abnormal memory | adult stage, with Scer\GAL4^GMR13F02

(Guven-Ozkan et al., 2016)

abnormal memory | adult stage, with Scer\GAL4^Mz604

(Guven-Ozkan et al., 2016)

abnormal memory | adult stage, with Scer\GAL4^NP2492

(Guven-Ozkan et al., 2016)

abnormal memory | adult stage, with Scer\GAL4^Orco.PK

(Guven-Ozkan et al., 2016)

abnormal memory | adult stage, with Scer\GAL4^ple.PF

(Guven-Ozkan et al., 2016)

abnormal neurophysiology, with Scer\GAL4^GH146

(Guven-Ozkan et al., 2016)

viable, with Scer\GAL4^Tub.PU

(Fulga et al., 2015)

Phenotype Manifest In

adult antennal lobe projection neuron, with Scer\GAL4^GH146

(Guven-Ozkan et al., 2016)

Detailed Description

Statement

Reference

Ectopic expression of mir-980^{sponge.V2.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^elav-C155 enhances 3 min, 1 hr and 3 hr memory but not 9 hr or 24 hr memory in an aversive olfactory memory assay in adults compared to controls, they also display improved memory acquisition, showing increased 3 min memory index after training with 2,3,4 or 12 (but not 6) shocks. Normalizing the memory index scores to their initial performance measured immediately after training (3 min) shows increased 3 hr memory retention by mir-980^{sponge.V2.Scer\UAS.T:Disc\RFP-mCherry} expressing flies (subjected to 4 shocks during training) compared to controls (subjected to 6 shocks during training).

The enhanced 3 hr memory in an aversive olfactory memory assay is observed in flies expressing mir-980^{sponge.V2.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^elav-C155 (using Scer\GAL80^ts.αTub84B to limit the temporal expression) throughout both development and adulthood or adulthood only but not in flies expressing the transgene only during development.

Expression of mir-980^{sponge.V2.Scer\UAS.T:Disc\RFP-mCherry} under the control of any of the following drivers: Scer\GAL4^Orco.PK, Scer\GAL4^GH146, Scer\GAL4^ey-OK107, Scer\GAL4[GMR13F02] Scer\GAL4^c316, Scer\GAL4^ple.PF, Scer\GAL4^Mz604 or Scer\GAL4^NP2492 results in enhanced 3 hr memory in an aversive olfactory memory assay in adults compared to controls; expression under the control of either Scer\GAL4^Gad1.3.098 or Scer\GAL4^GMR31F10 does not lead to memory enhancement. Expression of mir-980^{sponge.V2.Scer\UAS.T:Disc\RFP-mCherry} using any of the following: Scer\GAL4^GH146, Scer\GAL4^ey-OK107 or Scer\GAL4^238Y however leads to increased odor and/or shock sensitivity. Only expression under the Scer\GAL4^GMR13F02 or Scer\GAL4^elav-C155 leads to 3 hr memory enhancement without significantly changing either shock or odor avoidance compared to controls.

Ectopic expression of mir-980^{sponge.V2.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^GH146 increases excitability (mean firing frequency) of the projection neurons in the adult optic lobe compared to controls.

(Guven-Ozkan et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^elav-C155, mir-980^{sponge.V2.UAS.mCherry} has abnormal memory | adult stage phenotype, suppressible by Rbfox1^KK109100, Scer\GAL4^elav-C155

(Guven-Ozkan et al., 2016)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The increased 3 hr memory in an aversive olfactory assay characteristic for adult expressing mir-980^{sponge.V2.Scer\UAS.T:Disc\RFP-mCherry} under the control of Scer\GAL4^elav-C155 is fully suppressed by co-expression of Rbfox1^KK109100 RNAi. The memory index measured in flies expressing both transgenes is comparable to that observed in flies expressing Rbfox1^KK109100 alone and below the control levels, indicating that mir-980 is epistatic to Rbfox1 in memory control.

(Guven-Ozkan et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

61465

w^*; P{UAS-mCherry.mir-980.sponge.V2}attP40/CyO; P{UAS-mCherry.mir-980.sponge.V2}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mir-980^{sponge.V2.Scer\UAS.T:Disc\RFP-mCherry}

mir-980^{sponge.V2.UAS.mCherry}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close