FlyBase Allele Report: Dmel\Nrg[167.UAS]

General Information

Symbol

Dmel\Nrg^167.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0147729

Feature type

allele

Associated gene

Dmel\Nrg

Associated Insertion(s)

Carried in Construct

P{UAS-nrg¹⁶⁷}

Also Known As

UAS-nrg¹⁶⁷, UAS-NRG-167

Key Links

Transgenic product class

wild_type

(Islam et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Islam et al., 2003)

Mutagen

in vitro construct

(Islam et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-nrg¹⁶⁷}

Cytology

Description

The MtnA promoter is replaced with UASt sequences.

(Islam et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Nrg

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult insulin secreting cell of pars intercerebralis, with Scer\GAL4^repo.PL

(Clements et al., 2021)

adult posterior midgut epithelium, with Scer\GAL4^bun-GSG5961

(Resnik-Docampo et al., 2021)

adult posterior midgut epithelium, with Scer\GAL4^Su(H).GBE

(Resnik-Docampo et al., 2021)

neurite | adult stage, with Scer\GAL4^repo.PL

(Clements et al., 2021)

ocellar nerve, with Scer\GAL4^MS1075

(Kristiansen et al., 2005)

wing, with Nrg^l3, Scer\GAL4^MS1075

(Islam et al., 2004)

wing, with Scer\GAL4^MS1075

(Islam et al., 2004)

wing & sensory neuron | ectopic, with Scer\GAL4^MS1075

(Islam et al., 2004)

wing vein L1 & wing nerve, with Scer\GAL4^MS1075

(Islam et al., 2004)

wing vein L3 & wing nerve, with Scer\GAL4^MS1075

(Islam et al., 2004)

Detailed Description

Statement

Reference

Scer\GAL4^MS1075-driven expression of Nrg^167.Scer\UAS results in some weak alterations in ocellar sensory system axon guidance.

(Kristiansen et al., 2005)

Overexpression of Nrg^167.Scer\UAS in wing sensory neurons and two wing epithelial layers, mediated by Scer\GAL4^MS1075, causes a reduction in wing size and the appearance of ectopic sensory neurons. Additionally, there are severe axonal pathfinding defects including the distal extension of L1 axons, the absence of the proximal projection where the L1 and L3 nerve join in wild type, the clumping together of L1 axons and the penetration of the wing epithelium by the L1 nerve, an aberrant process which can extend beyond the wing tissue. Expression of Nrg^167.Scer\UAS under Scer\GAL4^MS1075 in a Nrg^l3 background results in the same phenotype.

(Islam et al., 2004)

When Nrg^{180.I.Scer\UAS} is driven by Scer\GAL4^GMR.PF no effects are seen on the number of photoreceptor cells or cone cells present in the eye.

(Islam et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Nrg^167.UAS, Scer\GAL4^bun-GSG5961 has increased occurrence of cell division | adult stage phenotype, suppressible by Egfr^DN.UAS.cBa, Scer\GAL4^bun-GSG5961

(Resnik-Docampo et al., 2021)

Phenotype Manifest In

Suppressed by

Statement

Reference

Nrg^167.UAS, Scer\GAL4^bun-GSG5961 has adult posterior midgut epithelium phenotype, suppressible by Egfr^DN.UAS.cBa, Scer\GAL4^bun-GSG5961

(Resnik-Docampo et al., 2021)

Nrg^167.UAS, Nrg^l3, Scer\GAL4^MS1075 has wing phenotype, suppressible by Egfr[+]/Egfr^t1

(Islam et al., 2004)

Nrg^167.UAS, Scer\GAL4^MS1075 has wing phenotype, suppressible by Egfr[+]/Egfr^t1

(Islam et al., 2004)

Nrg^167.UAS, Scer\GAL4^MS1075 has wing phenotype, suppressible by Egfr^t1

(Islam et al., 2004)

Enhancer of

Statement

Reference

Nrg^167.UAS, Scer\GAL4^GMR.PF is an enhancer of cone cell phenotype of Scer\GAL4^GMR.PF, ed^UAS.cBa

(Islam et al., 2003)

Nrg^167.UAS, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, ed^UAS.cBa

(Islam et al., 2003)

Nrg^167.UAS, Scer\GAL4^GMR.PF is an enhancer of eye photoreceptor cell phenotype of Scer\GAL4^GMR.PF, ed^UAS.cBa

(Islam et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The defective wing phenotype of Nrg^167.Scer\UAS; Scer\GAL4^MS1075 mutants is partially suppressed in a Egfr^t1 or Egfr^t1/+ background. Egfr^t1 also suppresses the wing phenotype when Nrg^167.Scer\UAS is expressed in a Nrg^l3 background.

(Islam et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Nrg^167.UAS/Scer\GAL4^repo.PL rescues Nrg⁸⁴⁹

(Clements et al., 2021)

Partially rescues

Nrg^167.UAS/Scer\GAL4^c316 partially rescues Nrg^ibx

(Carhan et al., 2005)

Nrg^167.UAS/Scer\GAL4^Mef2.247 partially rescues Nrg^ibx

(Carhan et al., 2005)

Fails to rescue

Nrg^167.UAS/Scer\GAL4^Ilp2.PR fails to rescue Nrg⁸⁴⁹

(Clements et al., 2021)

Nrg^167.UAS/Scer\GAL4^30Y fails to rescue Nrg^ibx

(Carhan et al., 2005)

Nrg^167.UAS/Scer\GAL4^Tab2-201Y fails to rescue Nrg^ibx

(Carhan et al., 2005)

Nrg^167.UAS/Scer\GAL4^MS1075 fails to rescue Nrg^l3

(Kristiansen et al., 2005)

Comments

Expression of Nrg^167.Scer\UAS under the control of Scer\GAL4^c316 results in substantial rescue of the Nrg^ibx central brain phenotype.

Expression of Nrg^167.Scer\UAS under the control of either Scer\GAL4^Mef2.247, Scer\GAL4^30Y or Scer\GAL4^Tab2-201Y does not appear to rescue the Nrg^ibx central brain phenotype.

Expression of Nrg^167.Scer\UAS under the control of either Scer\GAL4^Mef2.247 or Scer\GAL4^c316 results in a significant increase in the sexual receptivity of Nrg^ibx females, while expression under the control of Scer\GAL4^Tab2-201Y has no significant effect.

Expression of Nrg^167.Scer\UAS under the control of Scer\GAL4^30Y results in a small but significant decrease in the sexual receptivity of Nrg^ibx females.

(Carhan et al., 2005)

Scer\GAL4^MS1075-driven expression of Nrg^167.Scer\UAS in Nrg^l3 mutant animals shows a synergistic increase in the expressivity of OP axon alterations (i.e. axonal alterations are much more severe in those individuals that display the phenotype).

(Kristiansen et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

24172

w^*; P{UAS-nrg¹⁶⁷}2/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Nrg^167.Scer\UAS

Nrg^167.UAS

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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