FlyBase Allele Report: Dmel\amn[chpd]

General Information

Symbol

Dmel\amn^chpd

Species

D. melanogaster

Name

cheapdate

FlyBase ID

FBal0090484

Feature type

allele

Associated gene

Dmel\amn

Associated Insertion(s)

P{PZ}amn^chpd

Carried in Construct

Key Links

Allele class

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Moore et al., 1998)

Progenitor genotype

Associated Insertion(s)

P{PZ}amn^chpd

Cytology

Description

Insertion of a P{PZ} element in the open reading frame, C-terminal to the PACAP and GHRH homology regions.

(Moore et al., 1998)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Moore et al., 1998)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | drug conditional

(Wolf et al., 2002)

abnormal memory

(Yu et al., 2006, Yu et al., 2005)

chemical sensitive

(Wolf et al., 2002)

chemical sensitive | recessive

(Moore et al., 1998)

Phenotype Manifest In

Detailed Description

Statement

Reference

amn^chpd mutants do not exhibit any behavioral memory at 24 hours and only a small amount is present at 9 hours after conditioning. Unlike wild-type, there is no significant increase in odor-evoked calcium influx into the α branch of the mushroom body neurons in amn^chpd mutants at either 9 or 24 hours after conditioning.

(Yu et al., 2006)

amn^chpd flies have a defective olfactory memory. These flies fail to produce the increased calcium influx seen in the dorsal paired medial neurons of wild-type flies at 30 minutes after a conditioning of 3s exposure to odour, followed by a 60s odour stimulus applied simultaneously with 12 electric-shock pulses.

(Yu et al., 2005)

amn^chpd mutant flies show similar response profiles to wild-type flies in response to ethanol: flies have an initial startle response, a brief moment of quiescence followed by a sustained period of hyperactivity and a reduction in locomotor speed leading to eventual immobility. However, the amn^chpd mutants have a more intense hyperactive phase and reach maximal hyperactivity more quickly, leading to a faster onset of immobility. During the hyperactive phase, amn^chpd mutants initiate activity bouts less often than wild-type flies, but stay active for longer and spend more time moving fast. As these mutants begin to sedate, their bout frequency actually increases but their bout length is shorter.

(Wolf et al., 2002)

Hemizygous males and homozygous females show increased sensitivity to ethanol; they elute from an inebriometer with a mean elution time (MET) of approximately 15 minutes compared with an MET of 20 minutes for wild-type controls. amn^chpd flies show normal geotaxis and locomotor activity. Ethanol absorption and metabolism is normal in these flies. The ethanol-sensitive phenotype is reversed by treatment of the flies with forskolin (an adenylate cyclase activator) for 2 hours. A 4 hour treatment with forskolin further reduces ethanol sensitivity of amn^chpd flies compared with control flies. Treatment of amn^chpd flies with a cAMP analog that activates PKA also reverses the ethanol-sensitive phenotype. Treatment of amn^chpd flies with an antagonist of cAMP that inhibits PKA partially reverses the ethanol-sensitive phenotype.

(Moore et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

amn^chpd has chemical sensitive | recessive phenotype, suppressible by Adcy1^rut-2080

(Moore et al., 1998)

Enhancer of

Statement

Reference

amn^chpd is an enhancer of chemical sensitive phenotype of Arf6^NP5226/Arf6^EP2612

(Peru Y Colón de Portugal et al., 2012)

Suppressor of

Statement

Reference

amn^chpd is a suppressor of chemical sensitive phenotype of Adcy1^rut-2080

(Moore et al., 1998)

Other

Statement

Reference

rut[+]/Adcy1^rut-1, amn^chpd has abnormal thermotaxis | dominant phenotype

(Hong et al., 2008)

Pka-C1[+]/Pka-C1^B10, amn^chpd has abnormal thermotaxis | dominant phenotype

(Hong et al., 2008)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The sensitivity to ethanol-induced sedation seen in Arf51F^NP5226/Arf51F^EP2612 flies is enhanced if they also carry amn^chpd.

(Peru Y Colón de Portugal et al., 2012)

Double heterozygotes for amn^chpd and rut¹ show an inability to find a preferred temperature, with temperature preference spread across a wide gradient.

Double heterozygotes for amn^chpd and Pka-C1^B10 show an inability to find a preferred temperature, with temperature preference spread across a wide gradient.

(Hong et al., 2008)

amn^chpd rut²⁰⁸⁰ double mutants do not show increased sensitivity to ethanol in an inebriometer assay, although each single mutant does show increased sensitivity to ethanol in an inebriometer assay.

(Moore et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

amn¹

(Moore et al., 1998)

amn^EX

(Moore et al., 1998)

amn^X8

(Moore et al., 1998)

amn^28A

(Moore et al., 1998)

Rescued by

amn^chpd is rescued by amn^UAS.cWa/Scer\GAL4^c316

(Yu et al., 2005)

amn^chpd is rescued by amn^hs.PM

(Moore et al., 1998)

Comments

The ethanol sensitive phenotype is rescued by amn^hs.PM expressed using heat shock. Full rescue of the ethanol-sensitive phenotype is seen in animals given a daily heat shock throughout development, and is still seen 48 hours after the last heat shock, but the phenotype has reverted to ethanol-sensitivity 72 hours after the last heat shock. Three heat shocks at 24 hour intervals in adult flies is also sufficient to fully rescue the ethanol-sensitive phenotype.

(Moore et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26499

P{PZ}amn^chpd; P{neoFRT.clipped}40A P{FRT(w^hs).clipped}G13; ry⁵⁰⁶

Notes on Origin

Discoverer

Selected as: Altered ethanol sensitivity in an inebriometer assay.

(Moore et al., 1998)

Comments

Precise excision of the P{PZ} element restores the wild-type phenotype.

(Moore et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Amn^chpd

(Peru Y Colón de Portugal et al., 2012)

amn^chpd

(Hong et al., 2008, Yu et al., 2006, Yu et al., 2005)

Name Synonyms

Cheapdate

(Laakso et al., 2002)

cheapdate

(Hashimoto et al., 2002, Manev et al., 2000, Moore et al., 1998, Small and Bloom, 1998)

Secondary FlyBase IDs

References (12)

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