Arc1^esm18 mutants display increased larval floating in a density-based assay for body fat content, but no changes in the accumulation of lipids in the fat body, gut, imaginal tissues or eonocytes, and no effect on food consumption or physical activity.

Activation of neurons expressing Scer\GAL4^E347 in Arc1^esm18 mutants results in a large, significant decrease in larval floating in a density-based assay for body fat content as compared to controls.

Arc1^esm18 mutant flies exhibit significantly higher survival rates at both 48 hours and 72 hours after food withdrawal.

Arc1^esm18 mutant flies do not exhibit changes in longevity or average weight compared to revertant controls. There is no evidence of alterations in trehalose or glucose levels in mutants compared to controls.

While total locomotor activity of Arc1^esm18 mutant flies is indistinguishable from controls, the light/dark distribution of this activity is different. Arc1^esm18 animals are slightly, but significantly, more active in the night and less active during the day. This difference in activity is accounted for by differences in the distribution of sleep. Arc1^esm18 flies sleep more during the day, but are awake a greater part of the night. Total sleep levels are not altered.

Arc1^esm18 animals show a significant decrease in activity after 12 hours of starvation compared to the average activity of fed flies. Mutants also exhibit episodes of hyperlocomotion immediately before death.

No significant differences are found between amplitude and frequency of Arc1^esm18 and control mEJPs recorded in the presence of 1.5mM external calcium. Evoked activity is also normal in Arc1^esm18 mutants as measured by EJP amplitude after single pulse stimulation in 0.1, 0.2, 0.4 and 1.5mM calcium.

Under high-frequency stimulation (10Hz) Arc1^esm18 mutant neuromuscular junctions (NMJs) show a reduction of about 30% from baseline after 5 mins of stimulation, as in controls. This indicates that Arc1 is not required for normal basal transmission at the NMJ.

There are no obvious differences in either the development of potentiation or it's decay in Arc1^esm18 mutants compared to controls.

The number of boutons at the Arc1^esm18 mutant NMJs is not significantly different to controls.

Initial memory and it's decay are not affected in Arc1^esm18 mutants compared to controls. In a courtship assay, where a Arc1^esm18 mutant male is paired with a mated females for 1 hour, then tested for his ability to be conditioned by testing with a virgin either 10 minutes or 2 hours after training, no differences to controls are observed.

Arc1^esm18 mutant flies show no difference in circadian rhythm to control flies.