FlyBase Allele Report: Dmel\mRpL50[GD4975]

General Information

Symbol

Dmel\mRpL50^GD4975

Species

D. melanogaster

Name

FlyBase ID

FBal0210006

Feature type

allele

Associated gene

Dmel\mRpL50

Associated Insertion(s)

Carried in Construct

P{GD4975}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4975}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4975

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of primary ovarian insufficiency

CEA with mRpL50^KK111673

(Bakhshalizadeh et al., 2023)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | oogenesis, with Scer\GAL4^NP1624, mRpL50^KK111673

(Bakhshalizadeh et al., 2023)

abnormal cell number | spermatogenesis, with Scer\GAL4^NP1624, mRpL50^KK111673

(Bakhshalizadeh et al., 2023)

abnormal developmental rate | RU486 conditional, with Scer\GAL4^da.Switch.PT

(Rera et al., 2010)

decreased size | adult stage, with Scer\GAL4^NP1624, mRpL50^KK111673

(Bakhshalizadeh et al., 2023)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

short lived | female | RU486 conditional, with Scer\GAL4^da.Switch.PT

(Rera et al., 2010)

short lived | male | RU486 conditional, with Scer\GAL4^da.Switch.PT

(Rera et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

egg chamber, with Scer\GAL4^NP1624, mRpL50^KK111673

(Bakhshalizadeh et al., 2023)

female germline cell | adult stage | increased number, with Scer\GAL4^NP1624, mRpL50^KK111673

(Bakhshalizadeh et al., 2023)

female germline cyst, with Scer\GAL4^NP1624, mRpL50^KK111673

(Bakhshalizadeh et al., 2023)

germinal proliferation center hub, with Scer\GAL4^NP1624, mRpL50^KK111673

(Bakhshalizadeh et al., 2023)

male germline cell | adult stage | increased number, with Scer\GAL4^NP1624, mRpL50^KK111673

(Bakhshalizadeh et al., 2023)

ovary, with Scer\GAL4^NP1624, mRpL50^KK111673

(Bakhshalizadeh et al., 2023)

spermatocyte | decreased number, with Scer\GAL4^NP1624, mRpL50^KK111673

(Bakhshalizadeh et al., 2023)

spermatogonium | increased number, with Scer\GAL4^NP1624, mRpL50^KK111673

(Bakhshalizadeh et al., 2023)

testis, with Scer\GAL4^NP1624, mRpL50^KK111673

(Bakhshalizadeh et al., 2023)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Adults expressing mRpL50^GD4975 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression of mRpL50^GD4975 under the control of Scer\GAL4^da.Switch.PT in the presence of 20μ/ml RU486 results in a prolonged developmental phase compared to controls.

Expression of mRpL50^GD4975 under the control of Scer\GAL4^da.Switch.PT in the presence of 20μ/ml RU486 results in a significantly reduced weight in male flies.

Expression of mRpL50^GD4975 under the control of Scer\GAL4^da.Switch.PT in the presence of 20μ/ml RU486 significantly reduces adult lifespan compared to controls in both males and females.

(Rera et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in adult/pharate lethality or late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 20-30% or 30-40% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4975

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v15199

w¹¹¹⁸; P{GD4975}v15199

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4975

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mRpL50^GD4975

mRpL50^v15199

(Rera et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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