FlyBase Allele Report: Dmel\nAChRα4[GD3904]

General Information

Symbol

Dmel\nAChRα4^GD3904

Species

D. melanogaster

Name

FlyBase ID

FBal0210161

Feature type

allele

Associated gene

Dmel\nAChRα4

Associated Insertion(s)

Carried in Construct

P{GD3904}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD3904}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD3904

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal sleep, with Scer\GAL4^{nAChRα4.ryeP}

(Shi et al., 2014)

partially lethal - majority die, with Scer\GAL4^Tub.PU

(Syrzycka et al., 2019)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

(Tsubouchi et al., 2012)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expressing nAChRα4^GD3904 under the control of Scer\GAL4^Tub.PU (and Dicer-2 for more efficient RNAi) leads to partial lethality in females and, depending on the insertion, leads to either semi-lethality or full lethality in males.

(Syrzycka et al., 2019)

Scer\GAL4^{nAChRα4.ryeP}-mediated expression of nAChRα4^GD3904 (in the presence of a 'UAS-Dicer2' transgene) reduces total sleep length.

(Shi et al., 2014)

Expression of nAChRα4^GD3904 under the control of Scer\GAL4^elav.PU does not confer resistance to chronic nicotine-induced locomotor hyperactivity.

(Ren et al., 2012)

Adults expressing nAChRα4^GD3904 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of nAChRα4^GD3904 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD3904} insertion line used.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development