FlyBase Allele Report: Dmel\p53[GD11134]

General Information

Symbol

Dmel\p53^GD11134

Species

D. melanogaster

Name

FlyBase ID

FBal0210285

Feature type

allele

Associated gene

Dmel\p53

Associated Insertion(s)

Carried in Construct

P{GD11134}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11134}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11134

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of testicular cancer

CEA

(Napoletano et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of testicular cancer

is NOT exacerbated by HtrA2^DF1

(Napoletano et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | embryonic stage, with Scer\GAL4^{VP16.nanos.UTR}

(Tarayrah-Ibraheim et al., 2021)

decreased cell death | spermatogenesis, with Scer\GAL4^nanos.PU

(Napoletano et al., 2017)

hyperplasia | spermatogenesis, with Scer\GAL4^nanos.PU

(Napoletano et al., 2017)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

primordial germ cell | embryonic stage, with Scer\GAL4^{VP16.nanos.UTR}

(Tarayrah-Ibraheim et al., 2021)

spermatogonium, with Scer\GAL4^nanos.PU

(Napoletano et al., 2017)

testis | adult stage, with Scer\GAL4^nanos.PU

(Napoletano et al., 2017)

Detailed Description

Statement

Reference

The expression of p53^GD11134 under the control of Scer\GAL4^nos.PU results in a severe increase in the proportion of hyperplastic testes as compared to controls; these testes exhibit a significant decrease in necrosis at their apical tip (as shown by the number of spermatogonial cysts bearing TUNEL-positive cells), as compared to controls.

(Napoletano et al., 2017)

Adults expressing p53^GD11134 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11134

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^nanos.PU, p53^GD11134 has hyperplasia | spermatogenesis phenotype, non-enhanceable by HtrA2^DF1/HtrA2[+]

(Napoletano et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, p53^GD11134 has abnormal cell number | embryonic stage phenotype, suppressible by AIF^UAS.Tag:MYC, Scer\GAL4^{VP16.nanos.UTR}

(Tarayrah-Ibraheim et al., 2021)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^nanos.PU, p53^GD11134 has testis | adult stage phenotype, non-enhanceable by HtrA2^DF1/HtrA2[+]

(Napoletano et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^{VP16.nanos.UTR}, p53^GD11134 has primordial germ cell | embryonic stage phenotype, suppressible by AIF^UAS.Tag:MYC, Scer\GAL4^{VP16.nanos.UTR}

(Tarayrah-Ibraheim et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

The increased proportion of hyperplastic testes resulting from the expression of p53^GD11134 under the control of Scer\GAL4^nos.PU is not enhanced by HtrA2^DF1 heterozygosity.

(Napoletano et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v45139

w¹¹¹⁸; P{GD11134}v45139

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11134

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Dp53^IR

(Napoletano et al., 2017)

p53^GD11134

(Napoletano et al., 2017)

p53^Ri

(Tarayrah-Ibraheim et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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