UASt regulatory sequences drive expression of an inverted repeat.
In males expressing qtcGD8202 pan-neuronally, courtship of females is significantly decreased while male courtship is significantly increased, compared to controls.
Expression of qtcGD8202 in the mushroom bodies under the control of Scer\GAL4Mef2.247 abolishes male discrimination in males.
Targeting expression of qtcGD8202 to the most chemosensory peripheral neurons (under the control of Scer\GAL4GH146) does not affect male discrimination.
Expression of qtcGD8202 in various subsets of the peripheral taste neurons known to be involved in the sex pheromone response (under the control of one of Scer\GAL4Gr66a.PD, Scer\GAL4Gr68a.PB, Scer\GAL4Gr32a.3.776, or Scer\GAL4Gr33a-GAL4) results in high levels of sex discrimination in males. No alteration in female mating behavior is detected.
Adults expressing qtcGD8202 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.
Depending on the insertion line used, expression under the control of Scer\GAL4Mef2.PR can result in viable flies or adult/pharate lethality.
Expression under the control of Scer\GAL4pnr-MD237 may result in lethality, depending on the insertion line used.
Expression under the control of Scer\GAL4pnr-MD237 results in extra bristles on the notum in 30-40% of the Scer\GAL4pnr-MD237 expression domain.
Expression under the control of Scer\GAL4pnr-MD237 results in a colour difference between the central Scer\GAL4pnr-MD237 expression domain of the notum and the surrounding lateral region in 70-80% of the Scer\GAL4pnr-MD237 expression domain.
Expression under the control of Scer\GAL4pnr-MD237 results in the absence of 20-30% of the Scer\GAL4pnr-MD237-expressing area of the notum.