FlyBase Allele Report: Dmel\shakB[GD12666]

General Information

Symbol

Dmel\shakB^GD12666

Species

D. melanogaster

Name

FlyBase ID

FBal0210567

Feature type

allele

Associated gene

Dmel\shakB

Associated Insertion(s)

Carried in Construct

P{GD12666}

Also Known As

shakB RNAi, inx8 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12666}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12666

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

(Tsubouchi et al., 2012)

lethal, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

sarcomere, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Z disc, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

Detailed Description

Statement

Reference

Expression of shakB^GD12666 under the control of Scer\GAL4^ey-OK107 in adults does not significantly affect 3 hour aversive olfactory memory or the residual anaesthesia-resistant memory (ARM) after cold-shock, compared to controls; expression under the control of Scer\GAL4^VT000765 also does not affect ARM, compared to controls.

(Shyu et al., 2019)

Expression of shakB^GD12666 under the control of Scer\GAL4^17d, the split-GAL4 pair of Scer\GAL4^DBD.R41C07 and Hsap\RELA^AD.R13F02, the split-GAL4 pair of Scer\GAL4^DBD.R89B01 and Hsap\RELA^AD.R13F02, or the split-GAL4 pair of Scer\GAL4^DBD.R14C08 and Hsap\RELA^AD.R12C11, does not affect visual short term memory following training with pain stimulus in adult flies, compared to controls.

(Liu et al., 2016)

Expression of shakB^GD12666 under the control of Scer\GAL4^tj.PU (along with expression of UAS-Dcr2 to increase RNAi efficiency) does not cause sterility.

(Smendziuk et al., 2015)

Expression of shakB^GD12666 in both the anterior paired lateral (APL) and dorsal paired medial (DPM) neurons in the mushroom body (using the Scer\GAL4^GH146 and Scer\GAL4^c316 drivers simultaneously, and in the presence of Dcr-2^Scer\UAS.cDa to increase RNAi efficiency) has no effect on 3 hour memory after associative conditioning.

(Wu et al., 2011)

Adults expressing shakB^GD12666 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in embryonic lethality or undefined lethality.

Expression under the control of Scer\GAL4^Mef2.PR results in grossly normal larval body wall muscles.

Expression under the control of Scer\GAL4^Mef2.PR results in sarcomeres with discontinuous Z-lines in the larval body wall muscles.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12666

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^da.PU/shakB^GD12666, Scer\GAL80^ts.αTub84B is a suppressor | heat sensitive of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Scer\GAL4^54C/shakB^GD12666 is a non-suppressor of adult optic lobe glial cell | cell non-autonomous phenotype of ND-42^ninaE.GD6220

(Liu et al., 2017)

Scer\GAL4^54C/shakB^GD12666 is a non-suppressor of lipid droplet | cell non-autonomous | adult stage phenotype of ND-42^ninaE.GD6220

(Liu et al., 2017)

Scer\GAL4^elav.PLu/shakB^GD12666 is a non-suppressor of adult optic lobe glial cell | cell non-autonomous phenotype of ND-42^ninaE.GD6220

(Liu et al., 2017)

Scer\GAL4^elav.PLu/shakB^GD12666 is a non-suppressor of lipid droplet | cell non-autonomous | adult stage phenotype of ND-42^ninaE.GD6220

(Liu et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The accumulation of lipid droplets in glial cells in the vicinity of adult photoreceptor neurons expressing ND-42^ninaE.GD6220 is not suppressed by either Scer\GAL4^54C-driven (glial) or Scer\GAL4^elav.PLu-driven (neuronal) expression of shakB^GD12666.

(Liu et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v26801