FlyBase Allele Report: Dmel\Or67d[GAL4-2]

General Information

Symbol

Dmel\Or67d^GAL4-2

Species

D. melanogaster

Name

FlyBase ID

FBal0210949

Feature type

allele

Associated gene

Dmel\Or67d

Associated Insertion(s)

TI{GAL4}Or67d^GAL4-2

Carried in Construct

Also Known As

Or67d², Or67d^GAL4, pOR67d-GAL4

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

ends-in gene targeting

(Kurtovic et al., 2007, Kurtovic et al., 2007)

I-SceI

(Kurtovic et al., 2007)

FLPase

(Kurtovic et al., 2007)

I-CreI

(Kurtovic et al., 2007)

Progenitor genotype

Associated Insertion(s)

TI{GAL4}Or67d^GAL4-2

Cytology

Description

The Or67d open reading frame has been replaced by sequence encoding a GAL4 driver flanked on each side by loxP sites.

(Kurtovic et al., 2007)

Allele components

Component

Use(s)

Inserted element

TI{GAL4}

promoter trap

Encoded product / tool

GAL4

binary expression system - driver

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior

(Kurtovic et al., 2007, Kurtovic et al., 2007)

abnormal neurophysiology

(Ronderos et al., 2014, Kurtovic et al., 2007)

abnormal smell perception

(Ronderos et al., 2014)

Phenotype Manifest In

antennal trichoid sensillum at1

(Ronderos et al., 2014)

trichoid sensillum of antennal segment 3

(Kurtovic et al., 2007)

Detailed Description

Statement

Reference

In contrast to wild-type, flies homozygous for Or67d^GAL4-2 have no spontaneous firing of olfactory neurons within a single at1 trichoid sensillum. The mutant at1 neurons are insensitive to the male-specific pheromone 11-cis-vaccenyl acetate (cVA).

(Ronderos et al., 2014)

Or67d^GAL4-2 males paired with wild-type males show a significantly higher male-male courtship index than that of control males paired with wild-type males.

(Kurtovic et al., 2007)

The male pheromone cVA ([Z]-11-octadecenyl acetate) elicits a rapid and robust firing response in the T1 trichoid sensilla of control flies but not in those of Or67d^GAL4-2 flies. Male and female Or67d^GAL4-2 flies do not produce a detectable electroretinogram response to cVA (in contrast to controls), but they show a normal electroretinogram response to ethanol.

When paired with virgin females, Or67d^GAL4-2 males court at levels comparable to those of control males. However, when Or67d^GAL4-2 males are paired with wild-type males, they show a roughly threefold higher courtship activity than control males paired with wild-type males.

Or67d^GAL4-2 females mated to wild-type males show a higher latency to copulation than control females mated to wild-type males.

Virgin Or67d^GAL4-2 females are courted as vigorously as control females when paired with wild-type males.

(Kurtovic et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Or67d^GAL4-2, Or83c^UAS.cRa, Scer\GAL4^Or67d-GAL4-2 has abnormal neurophysiology phenotype

(Ronderos et al., 2014)

Or67d^GAL4-2, Or83c^UAS.cRa, Scer\GAL4^Or67d-GAL4-2 has abnormal smell perception phenotype

(Ronderos et al., 2014)

Phenotype Manifest In

Other

Statement

Reference

Or67d^GAL4-2, Or83c^UAS.cRa, Scer\GAL4^Or67d-GAL4-2 has antennal trichoid sensillum at1 phenotype

(Ronderos et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Flies homozygous for Or67d^GAL4-2 and expressing Scer\GAL4^Or67d-GAL4-2>Or83c^Scer\UAS.cRa show restored spontaneous activity in at1 neurons, but to a distinct higher average firing rate. The mutant neurons expressing Or83c^Scer\UAS.cRa remain insensitive to the male-specific pheromone 11-cis-vaccenyl acetate (cVA), similar to Or67d^GAL4-2 mutants.

(Ronderos et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Or67d^Z5499

(Jin et al., 2008)

Rescued by

Or67d^GAL4-2 is rescued by Or67d^UAS.cKa/Scer\GAL4^Or67d-GAL4-2

(Kurtovic et al., 2007)

Or67d^GAL4-2 is rescued by Or67d^UAS.cKa/Scer\GAL4^Or67d-GAL4-2

(Kurtovic et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

OR67d²

(Guo et al., 2022)

Or67d<up> GAL4</up>

(Ronderos et al., 2014)

Or67d²

(Hu et al., 2024, Ha et al., 2014, Jin et al., 2008)

Or67d^GAL4-2

Or67d^GAL4

(Ha et al., 2014, Ronderos et al., 2014, Kurtovic et al., 2007, Kurtovic et al., 2007, Kyriacou, 2007)

Or67d^GAL4[2]

(Kurtovic et al., 2007, Kurtovic et al., 2007)

pOr67d^Gal4

(Guo et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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